农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2008年
5期
40-42,123
,共4页
希尼尼根%申宏旺%关平原
希尼尼根%申宏旺%關平原
희니니근%신굉왕%관평원
Holstein-friesian cow%Interleukin-2%Cloning%Prokaryotic expression
[Objective] The aim of this study is to clone bovine interleukin-2 gene (IL-2) and observe its expression in prokaryotic cells. [Method] Bo-vine IL-2 gene was amplified from total RNA of peripheral blood lymphocytes of holstein-friesian cows by RT-PCR. Subsequently, the gene was cloned into pGEX-2T prokaryotic expression plasmid to construct recombinant, which was then transformed into Escherichia Coli BI21. After IPTG induction, SDS-PAGE analysis was conducted. [Result] A 500 bp target fragment corresponding with expectation was obtained by RT-PCR. The cloned gene successfully expressed fusion protein of about 43 kD in prokaryotic cells. [Conclusion] This study provided a theoretical and material basis for further researches on IL-2 gene.
