CAJ | 학술논문

丝黑穗病是影响我国高粱生产发展的主要病害之一,在高粱产区每年都有发生,发病率有时高达70 %,给生产造成很大损失,而选育抗病品种是最为有效的抗病策略.分别以高粱2381恢复系(抗病)、矮四恢复系(感病)的叶片为试材,采用CTAB法提取高粱基因组DNA,并应用RAPD筛选技术对高粱基因进行分子标记.在最佳的RAPD反应体系中共筛选了100个RAPD随机引物,筛选出来27个适宜引物,获得了稳定的RAPD扩增结果及多态性较好的DNA谱带.其中有6个引物对DNA扩增产生了差异谱带.
사흑수병시영향아국고량생산발전적주요병해지일,재고량산구매년도유발생,발병솔유시고체70 %,급생산조성흔대손실,이선육항병품충시최위유효적항병책략.분별이고량2381회복계(항병)、왜사회복계(감병)적협편위시재,채용CTAB법제취고량기인조DNA,병응용RAPD사선기술대고량기인진행분자표기.재최가적RAPD반응체계중공사선료100개RAPD수궤인물,사선출래27개괄의인물,획득료은정적RAPD확증결과급다태성교호적DNA보대.기중유6개인물대DNA확증산생료차이보대.
The head smut was one of the main disease in sorghum over the world, and one of primary diseases which has a long term influence on sorghum production in China.It happens in the area of sorghum production every year, sometimes the incidence percentage was up to 70% and there was big loss in sorghum production. However,selecting the resistant plant is the most efficient strategy. The paper exerts CTAB method to extract the genomic DNA of sorghum by using the 2381(resistant individual) and short IV(susceptible individual) and applies molecular marker to sorghm gene by RAPD. In the optimal RAPD reaction system, in total, 100 random primers were used to screen the markers linked to the resistance gene,and result in relative excellent polymorphic DNA, of which there are 27 proper primers and particularly 6 primers can get differential band.