CAJ | 학술논문

[目的]研究黄姜中自身转化薯蓣皂苷元的提取方法.[方法]基于黄姜中自身酶的作用,将黄姜在室温条件下分别保存15和30 d,使带有3个糖基的薯蓣皂苷在其自身酶作用下部分转化生成无糖基薯蓣皂苷元,并对黄姜自身转化薯蓣皂苷元进行提取、分离、检测,比较不同保存时间的提取效果.[结果]500 g黄姜在室温下保存15和30 d,经70%乙醇浸提分别得薯蓣总皂苷30.8和24.7 g,总皂苷得率分别为6.16%和4.94%,用大孔吸附树脂AB-8分离提纯后分别得薯蓣皂苷元6.3和11.7 g,苷元得率分别为1.26%和2.34%,继后利用D-280大孔吸附树脂脱色最终得薯蓣皂苷元分别为4.97和10.2 g,得率分别为0.99%和2.04%.[结论]保存30 d的黄姜,经大孔吸附树脂分离纯化后,其薯蓣皂苷元是保存15 d黄姜的2倍,经HPLC检测,其纯度为91%.
[목적]연구황강중자신전화서여조감원적제취방법.[방법]기우황강중자신매적작용,장황강재실온조건하분별보존15화30 d,사대유3개당기적서여조감재기자신매작용하부분전화생성무당기서여조감원,병대황강자신전화서여조감원진행제취、분리、검측,비교불동보존시간적제취효과.[결과]500 g황강재실온하보존15화30 d,경70%을순침제분별득서여총조감30.8화24.7 g,총조감득솔분별위6.16%화4.94%,용대공흡부수지AB-8분리제순후분별득서여조감원6.3화11.7 g,감원득솔분별위1.26%화2.34%,계후이용D-280대공흡부수지탈색최종득서여조감원분별위4.97화10.2 g,득솔분별위0.99%화2.04%.[결론]보존30 d적황강,경대공흡부수지분리순화후,기서여조감원시보존15 d황강적2배,경HPLC검측,기순도위91%.
[Objective] The aim was to research the extraction method of diosgenin through itself transforming in Dioscorea zingiberensis C.H. Wright. [Method] D. zingiberensis was preserved for 15 and 30 d resp. under the room temperature condition on base of the effect of itself enzyme in D. zingiberensis And then the diosgenin with 3 glycosyl was part transformed into non-glycosyl diosgenin under the effect of itself enzyme, the itself transformed diosgenin in D. zingiberensis was extracted, separated and measured and the extraction effect under the different preserve times were compared. [Result] When 500g D. Zingiberensis were preserved for 15 and 30 d under the room temperature resp., the diosgenin were got by 30.8 and 24.7 g resp. and the total saponin yield was 6.16 % and 4.94 % resp. through extracting by 70% ethanol; the diosgenin was got by 6.3 and 11.7 g resp. and the saponin yield was 1.26% and 2.34% after separation and purification by using macroporous absorption resin; and then the final diosgenin was 4.97 and 10.2g and its yield was 0.99% and 2.04% after decoloration by using D-280 macroporous absorption resin. [Conclusion] The diogenin in D. zingiberensis preserved at 30 d was 2 times of that preserved at 15 d after separation and purification by using macroporous absorption resin, and its purity was 91% by HPLC detection.