CAJ | 학술논문

目的:探讨组织工程仿生骨膜同种异体内成骨修复骨缺损及其血管化的可能性.方法:以猪小肠黏膜下层基质为支架复合体外培养的新西兰大白兔骨髓间充质干细胞构建组织工程仿生骨膜.2个月龄新西兰大白兔12只,制备双侧桡骨临界骨缺损模型.随机选一侧植入组织工程仿生骨膜,作为实验组;另一侧仅植入单纯小肠黏膜下层基质,作为对照组.植入后观察动物一般情况;植入4周时,以四环素荧光标记法、甲醛-墨汁灌注法,观察工程骨组织的血管化情况,同时以苏木精-伊红染色观察新骨组织形成.结果:术后动物饮食及日常行为基本正常;伤口无红肿、溢脓等.大体标本观察见实验侧骨缺损得到初步修复,而对照侧骨缺损未修复.四环素荧光标记及组织学染色均证明实验侧骨缺损处有新生骨组织;甲醛-墨汁灌注标本检测证明工程骨组织中有较丰富的血管形成.结论:所构建的组织工程仿生骨膜在同种异体体内可以成骨,并可血管化成活.
목적:탐토조직공정방생골막동충이체내성골수복골결손급기혈관화적가능성.방법:이저소장점막하층기질위지가복합체외배양적신서란대백토골수간충질간세포구건조직공정방생골막.2개월령신서란대백토12지,제비쌍측뇨골림계골결손모형.수궤선일측식입조직공정방생골막,작위실험조;령일측부식입단순소장점막하층기질,작위대조조.식입후관찰동물일반정황;식입4주시,이사배소형광표기법、갑철-묵즙관주법,관찰공정골조직적혈관화정황,동시이소목정-이홍염색관찰신골조직형성.결과:술후동물음식급일상행위기본정상;상구무홍종、일농등.대체표본관찰견실험측골결손득도초보수복,이대조측골결손미수복.사배소형광표기급조직학염색균증명실험측골결손처유신생골조직;갑철-묵즙관주표본검측증명공정골조직중유교봉부적혈관형성.결론:소구건적조직공정방생골막재동충이체체내가이성골,병가혈관화성활.
OBJECTIVE: To investigate the in vivo possibility of osteogenesis and angiogenesis of tissue-engineered periosteum in rabbits.METHODS: The marrow mesenchymal stem cells (MSCs) derived from New Zealand rabbits were adhered to small intestinal submucosa (SIS) to fabricate the tissue-engineered periosteum. Totally 12 New Zealand rabbits were received critical bone defect in bilateral radii to prepare models. The tissue-engineered periosteum was randomly implanted in one side of bone defect,and the other side was treated by SIS. At 4 weeks after operation, the angiogenesis of tissue engineered bone was detected by Tetracycline fluorescence microscopy and formaldehyde-ink perfusion method; simultaneously, the new bone formation was firmed by haematoxylin-eosin staining.RESULTS: Animals showed normal daily behaviors and non-infection wounds healing. The gross observation showed that bone defects in the experimental side were bridged with newly formed bone; while the defects of the control side were remained empty.Tetracycline fluorescence microscopy and hisotological examination could confirm the new bone tissue formation in the experimental side. The ink staining in new bone specimens suggested that there were abundant of neovasculization in tissue-engineered bone.CONCLUSION: Tissue-engineered periosteum can form new bone in allogenic rabbits and can be vascularized by some inherent mechanism for new bone tissue survivor.