华东师范大学学报（自然科学版）
華東師範大學學報（自然科學版）
화동사범대학학보（자연과학판）
JOURNAL OF EAST CHINA NORMAL UNIVERSITY(NATURAL SCIENCE)
2010年
2期
58-66
,共9页
钱丽娟%郭素珍%曹庆珍%刘俊奇%施华宏
錢麗娟%郭素珍%曹慶珍%劉俊奇%施華宏
전려연%곽소진%조경진%류준기%시화굉
甲状腺激素干扰物%非洲爪蟾%镉%变态
甲狀腺激素榦擾物%非洲爪蟾%鎘%變態
갑상선격소간우물%비주조섬%력%변태
thyroid disrupting chemicals (TDCs)%Xenopus laevis%cadmium%metamorphosis
将NF51阶段非洲爪蟾(Xenopus laevis)蝌蚪暴露于Cd~(2+)浓度为0.01,0.1和1 mg·L~(-1)的溶液中21 d,观测Cd~(2+)对爪蟾的生长、变态和甲状腺组织结构的影响.1 mg·L~(-1)Cd~(2+)暴露组爪蟾蝌蚪与对照组相比,7 d后,平均体重减少了0.39倍,体长和后肢长分别减少了0.17倍和0.27倍,发育阶段稍有延缓;21 d后,1 mg·L~(-1)Cd~(2+)暴露组爪蟾蝌蚪后肢长减小了0.11倍,延缓了1个发育阶段.其余各浓度组在7 d和21 d暴露后,体重、体长、后肢长和发育阶段与对照组无显著差异.21 d后,Cd~(2+)各浓度组甲状腺组织结构出现滤泡数目减少、胶质减少甚至空泡化等现象,1 mg·L~(-1)Cd~(2+)暴露组甲状腺滤泡之间还出现溶通现象.结果表明CdCl_2具有甲状腺激素干扰效应,并可能是由于该化合物直接作用于甲状腺组织引起.由此可见,爪蟾变态实验可以有效检测重金属盐的甲状腺激素干扰效应.
將NF51階段非洲爪蟾(Xenopus laevis)蝌蚪暴露于Cd~(2+)濃度為0.01,0.1和1 mg·L~(-1)的溶液中21 d,觀測Cd~(2+)對爪蟾的生長、變態和甲狀腺組織結構的影響.1 mg·L~(-1)Cd~(2+)暴露組爪蟾蝌蚪與對照組相比,7 d後,平均體重減少瞭0.39倍,體長和後肢長分彆減少瞭0.17倍和0.27倍,髮育階段稍有延緩;21 d後,1 mg·L~(-1)Cd~(2+)暴露組爪蟾蝌蚪後肢長減小瞭0.11倍,延緩瞭1箇髮育階段.其餘各濃度組在7 d和21 d暴露後,體重、體長、後肢長和髮育階段與對照組無顯著差異.21 d後,Cd~(2+)各濃度組甲狀腺組織結構齣現濾泡數目減少、膠質減少甚至空泡化等現象,1 mg·L~(-1)Cd~(2+)暴露組甲狀腺濾泡之間還齣現溶通現象.結果錶明CdCl_2具有甲狀腺激素榦擾效應,併可能是由于該化閤物直接作用于甲狀腺組織引起.由此可見,爪蟾變態實驗可以有效檢測重金屬鹽的甲狀腺激素榦擾效應.
장NF51계단비주조섬(Xenopus laevis)과두폭로우Cd~(2+)농도위0.01,0.1화1 mg·L~(-1)적용액중21 d,관측Cd~(2+)대조섬적생장、변태화갑상선조직결구적영향.1 mg·L~(-1)Cd~(2+)폭로조조섬과두여대조조상비,7 d후,평균체중감소료0.39배,체장화후지장분별감소료0.17배화0.27배,발육계단초유연완;21 d후,1 mg·L~(-1)Cd~(2+)폭로조조섬과두후지장감소료0.11배,연완료1개발육계단.기여각농도조재7 d화21 d폭로후,체중、체장、후지장화발육계단여대조조무현저차이.21 d후,Cd~(2+)각농도조갑상선조직결구출현려포수목감소、효질감소심지공포화등현상,1 mg·L~(-1)Cd~(2+)폭로조갑상선려포지간환출현용통현상.결과표명CdCl_2구유갑상선격소간우효응,병가능시유우해화합물직접작용우갑상선조직인기.유차가견,조섬변태실험가이유효검측중금속염적갑상선격소간우효응.
Xenopus laevis tadpoles (NF51) were exposed to cadmium chloride at the concentrations of 0.01, 0.1 and 1 mg·L~(-1)Cd~(2+) for 21 days.The changes of growth, metamorphosis and thyroid gland of X.laevis were observed.Compared with the control, the body weight (BW), snout to vent length (SVL) and hind limb length (HLL) were decreased by 0.39, 0.17 and 0.27 fold in 1 mg·L~(-1) treatment group after exposure for 7 days, respectively.The hind limb length (HLL) was decreased by 0.11 fold and the development showed one stage slower in 1 mg·L~(-1) treatment group after exposure for 21 days.And the other treatment groups did not show significant changes in BW, SVL, HLL or development stages after exposure for 7 and 21 days.Cadmium chloride treatment decreased the number of follicle, and induced the depletion of colloid and even vacuolation after exposure for 21 days.In addition, amalgamation of two or more follicles occurred in 1 mg·L~(-1) treatment group.Our results suggested that cadmium chloride had thyroid hormone disrupting effects and might disturb thyroid gland tissue directly.Therefore, AMA was further proved to be a valid tool to screen the thyroid hormone disrupting effect of heavy metal salt.
