CAJ | 학술논문

以阿魏菇NB-1为液体发酵菌种,在发酵培养液中添加阿魏的三氯甲烷萃取物,27.5℃培养7d,测定发酵液中漆酶、多酚氧化酶(PPO)、蛋白酶以及菌丝体中PPO酶活力.结果表明:阿魏三氯甲烷萃取物添加量为10、50、100mg/100mL时,发酵液中漆酶酶活力分别是对照的40、50、50倍;发酵液PPO酶活力分别是对照的4.43、30.14、43.14倍;菌丝体中PPO酶活力分别是对照的1.6、0.93、1.06倍,萃取物对菌丝体PPO酶活力产生了双重作用;发酵液蛋白酶酶活力分别为对照的0.99、0.85、0.67倍,表现出随阿魏三氯甲烷萃取物剂量增加,酶活力降低的趋势.
이아위고NB-1위액체발효균충,재발효배양액중첨가아위적삼록갑완췌취물,27.5℃배양7d,측정발효액중칠매、다분양화매(PPO)、단백매이급균사체중PPO매활력.결과표명:아위삼록갑완췌취물첨가량위10、50、100mg/100mL시,발효액중칠매매활력분별시대조적40、50、50배;발효액PPO매활력분별시대조적4.43、30.14、43.14배;균사체중PPO매활력분별시대조적1.6、0.93、1.06배,췌취물대균사체PPO매활력산생료쌍중작용;발효액단백매매활력분별위대조적0.99、0.85、0.67배,표현출수아위삼록갑완췌취물제량증가,매활력강저적추세.
In this study, Pleurotus ferulae NB- 1 was used for liquid fermentation. The chloroform soluble fraction of 95% ethanol extract from Fendafendaeoides root was added to fermentation culture medium of the swain. After 7 days of fermentation at 27.5 ℃,the resulting fermentation broth was harvested and separated into supematant and mycelia by centrifugation, and the activities of laccase, polyphenol oxidase (PPO) and protease in the supernatant and mycelial PPO were determined. Results indicated that the addition of the chloroform soluble fraction of 95% ethanol extract from Ferulaferulaeoides root at the levels of 10, 50 and 100 mg to 100 mL of fermentation culture medium resulted in 40-, 50- and 50-fold increases of laccase activity, 4.43-, 30.14- and 43.14-fold increases of PPO activity and 0.99-, 0.85- and 0.67-fold increase of protease in the supematant, and 1.6-, 0.93- and 1.06-fold increases of mycellal PPO activity, respectively when compared with no addition. These results demonstrate that the chloroform soluble fraction of 95% ethanol extract from Fendaferulaeoides root exhibits a promotion effect at low concentration and an inhibition effect on PPO activity at high concentration. Moreover, protease activity exhibits a decreasing trend in a dose-dependent manner.