中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2010年
4期
640-644
,共5页
高瞻%来丹丹%黄晓燕%褚茂平%施翔翔%张怀勤%杨德业
高瞻%來丹丹%黃曉燕%褚茂平%施翔翔%張懷勤%楊德業
고첨%래단단%황효연%저무평%시상상%장부근%양덕업
Paired box gene 8%Bcl2-like 14%室间隔缺损%细胞凋亡
Paired box gene 8%Bcl2-like 14%室間隔缺損%細胞凋亡
Paired box gene 8%Bcl2-like 14%실간격결손%세포조망
Paired box gene 8%Bcl2-like 14%Ventricular septal defects%Apoptosis
目的:寻找先天性心脏病相关基因-转录因子Pax-8的下游基因.方法:分别提取Pax-8基因敲除小鼠纯合子(Pax-8 KO~(-/-))和杂合子(Pax-8 KO~(+/-))的心脏总RNA,利用含31 802个小鼠基因的基因芯片检测两组小鼠基因表达水平,找出差异表达的基因,并经半定量RT-PCR和荧光实时定量PCR技术初步筛选出转录因子Pax-8的下游基因.结果:基因芯片检测发现,Pax-8 KO~(-/-) 组与Pax-8 KO~(+/-) 相比有25个基因表达下调,另有17个基因表达上调,差异基因涉及细胞周期及信号转导的调节因子,直接参与代谢的酶,以及核转录因子等.用半定量RT-PCR验证发现:Bcl2-like 14(Bcl2l14)基因在Pax-8 KO~(-/-) 组上调.定量RT-PCR亦证实在Pax-8 KO~(-/-)组Bcl2l14基因的表达水平较Pax-8 KO~(+/-) 组及Pax-8 KO~(+/+)(野生型)组分别上调2.07倍和2.23倍(P<0.01).结论:Bcl2l14基因为转录因子Pax-8的下游基因,可能在先天性心脏病室间隔缺损的发病机制中发挥重要作用.
目的:尋找先天性心髒病相關基因-轉錄因子Pax-8的下遊基因.方法:分彆提取Pax-8基因敲除小鼠純閤子(Pax-8 KO~(-/-))和雜閤子(Pax-8 KO~(+/-))的心髒總RNA,利用含31 802箇小鼠基因的基因芯片檢測兩組小鼠基因錶達水平,找齣差異錶達的基因,併經半定量RT-PCR和熒光實時定量PCR技術初步篩選齣轉錄因子Pax-8的下遊基因.結果:基因芯片檢測髮現,Pax-8 KO~(-/-) 組與Pax-8 KO~(+/-) 相比有25箇基因錶達下調,另有17箇基因錶達上調,差異基因涉及細胞週期及信號轉導的調節因子,直接參與代謝的酶,以及覈轉錄因子等.用半定量RT-PCR驗證髮現:Bcl2-like 14(Bcl2l14)基因在Pax-8 KO~(-/-) 組上調.定量RT-PCR亦證實在Pax-8 KO~(-/-)組Bcl2l14基因的錶達水平較Pax-8 KO~(+/-) 組及Pax-8 KO~(+/+)(野生型)組分彆上調2.07倍和2.23倍(P<0.01).結論:Bcl2l14基因為轉錄因子Pax-8的下遊基因,可能在先天性心髒病室間隔缺損的髮病機製中髮揮重要作用.
목적:심조선천성심장병상관기인-전록인자Pax-8적하유기인.방법:분별제취Pax-8기인고제소서순합자(Pax-8 KO~(-/-))화잡합자(Pax-8 KO~(+/-))적심장총RNA,이용함31 802개소서기인적기인심편검측량조소서기인표체수평,조출차이표체적기인,병경반정량RT-PCR화형광실시정량PCR기술초보사선출전록인자Pax-8적하유기인.결과:기인심편검측발현,Pax-8 KO~(-/-) 조여Pax-8 KO~(+/-) 상비유25개기인표체하조,령유17개기인표체상조,차이기인섭급세포주기급신호전도적조절인자,직접삼여대사적매,이급핵전록인자등.용반정량RT-PCR험증발현:Bcl2-like 14(Bcl2l14)기인재Pax-8 KO~(-/-) 조상조.정량RT-PCR역증실재Pax-8 KO~(-/-)조Bcl2l14기인적표체수평교Pax-8 KO~(+/-) 조급Pax-8 KO~(+/+)(야생형)조분별상조2.07배화2.23배(P<0.01).결론:Bcl2l14기인위전록인자Pax-8적하유기인,가능재선천성심장병실간격결손적발병궤제중발휘중요작용.
AIM: To investigate the downstream genes of the transcriptional factor Pax-8 related to cardiopathy. METHODS: The total RNA derived from the heart of Pax-8 KO~(-/-) and Pax-8 KO~(+/-) mice was extracted. Mouse genome DNA microarray containing 31 802 mouse oligonucleotides probes was used to investigate the differential expression between the Pax-8 KO~(-/-) and the Pax-8 KO~(+/-) mice hearts. The candidate genes were confirmed by RT-PCR and real time RT-PCR assay. RESULTS: Microarray results showed that, compared to the Pax-8 KO~(+/-) mice, 25 genes were down-regulated and 17 were up-regulated in the Pax-8 KO~(-/-) mice, concerning metabolize enzymes, cell signal conducting and nuclear transcript factors and so on. Bcl2-like 14 (Bcl2l14) was proved to be up-regulated by RT-PCR. Real time RT-PCR results revealed that Bcl2l14 in the Pax-8 KO~(-/-) mice was 2.07 and 2.23 fold as much as that in the Pax-8 KO~(+/-) and the Pax-8KO~(+/+) mice (P<0.01). CONCLUSION: The Bcl2l14 gene is one of the downstream genes of Pax-8 and probably plays an important role in the mechanism of ventricular septum defect.
