CAJ | 학술논문

目的探讨雌激素受体基因多态性与陕西地区慢性牙周炎易感性的关联.方法收集陕西地区109例慢性牙周炎患者和99例牙周健康对照组的颊黏膜拭子,用Chelex-100方法提取全基因组DNA,用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测雌激素α和β受体基因型分布.结果慢性牙周炎组与正常对照组在ER-α受体Xba Ⅰ基因型分布上有统计学差异,慢性牙周炎组XX型基因频率明显高于正常对照组,尤其在女性患者中此差异显著,男性患者中未见不同;ER-β受体Rsa Ⅰ和Alu Ⅰ基因型在患者组与对照组中分布未见差异.结论慢性牙周炎易感性与雌激素Xba Ⅰ基因型分布相关,汉族女性群体中ER-α受体XX基因型可能为慢性牙周炎的易感因子.
목적 탐토자격소수체기인다태성여협서지구만성아주염역감성적관련.방법 수집협서지구109례만성아주염환자화99례아주건강대조조적협점막식자,용Chelex-100방법제취전기인조DNA,용취합매련반응-한제성편단장도다태성(PCR-RFLP)기술검측자격소α화β수체기인형분포.결과 만성아주염조여정상대조조재ER-α수체Xba Ⅰ기인형분포상유통계학차이,만성아주염조XX형기인빈솔명현고우정상대조조,우기재녀성환자중차차이현저,남성환자중미견불동;ER-β수체Rsa Ⅰ화Alu Ⅰ기인형재환자조여대조조중분포미견차이.결론 만성아주염역감성여자격소Xba Ⅰ기인형분포상관,한족녀성군체중ER-α수체XX기인형가능위만성아주염적역감인자.
Objective To determine whether polymorphisms in the estrogen receptor (ER) genes are associated with chronic periodontitis in a Chinese population overall the Shanxi territory. Methods 109 patients with chronic periodontitis and 99 healthy controls were recruited in this study. Cenomic DNA was extracted from oral mucosa swab sample of each subject by the Chelex-100 method. Determination of the ER-α and -β polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), respectively. Results The analysis of the Xba I polymorphisms in ER-α gene revealed significant differences between patients and controls. The detection frequency of XX genotype was significantly higher in the chronic periodontitis patients than in control subjects. The difference between the female chronic periodontitis patients and healthy controls was statistically significant, but no difference was found between the male patients and controls. Nevertheless, no significant association was noted in the frequency of both Rsa I and Alu I polymorphisms in ER- β gene between chronic periodontitis and controls. Conclusion It is indicated that the ER-α XX genotype may be a susceptible indicator for chronic periodontitis in female Han Chinese subjects.