CAJ | 학술논문

背景:G_1期调节蛋白对调控增殖细胞的增殖周期具有重要作用,目前关于成骨细胞G_1期调节蛋白的研究甚少,绝经后骨质疏松与成骨细胞G_1期调节蛋白的关系更未被阐明.目的:观察去卵巢大鼠骨质疏松发病过程中成骨细胞G_1期调节蛋白的改变,探讨绝经后骨质疏松症的发病机制.方法:100只6月龄SD雌性大鼠,随机分成假手术组和模型组各50只,模型组进行双侧卵巢结扎切除术,假手术组除未行卵巢结扎切除外,其余步骤与手术组相同.所有大鼠于术后4,8,12,18,24周分批取材,每批每组各处死10只,取大鼠腰椎.运用免疫组织化学方法检测骨组织中成骨细胞G_1期调节蛋白Cyclin D1、CDK4、p21蛋白表达.结果与结论:Cyclin D1、CDK4蛋白阳性表达主要定位于骨小梁表面的成骨细胞.假手术组有Cyclin D1、CDK4蛋白阳性表达,模型组表达强于假手术组.p21蛋白阳性表达部位与Cyclin D1相似,假手术组和模型组均有明显阳性表达,但模型组的表达维持在较高水平,在各时期均明显高于同期假手术组(P<0.01).结果证实,去卵巢骨质疏松模型大鼠发病过程中,成骨细胞Cyclin D1、CDK4、p21蛋白出现明显高表达.提示成骨细胞增殖加快,同时成骨细胞周期受阻滞亦增多,成骨细胞数量相对不足,骨形成低于骨吸收,导致骨质疏松的发生.
배경:G_1기조절단백대조공증식세포적증식주기구유중요작용,목전관우성골세포G_1기조절단백적연구심소,절경후골질소송여성골세포G_1기조절단백적관계경미피천명.목적:관찰거란소대서골질소송발병과정중성골세포G_1기조절단백적개변,탐토절경후골질소송증적발병궤제.방법:100지6월령SD자성대서,수궤분성가수술조화모형조각50지,모형조진행쌍측란소결찰절제술,가수술조제미행란소결찰절제외,기여보취여수술조상동.소유대서우술후4,8,12,18,24주분비취재,매비매조각처사10지,취대서요추.운용면역조직화학방법검측골조직중성골세포G_1기조절단백Cyclin D1、CDK4、p21단백표체.결과여결론:Cyclin D1、CDK4단백양성표체주요정위우골소량표면적성골세포.가수술조유Cyclin D1、CDK4단백양성표체,모형조표체강우가수술조.p21단백양성표체부위여Cyclin D1상사,가수술조화모형조균유명현양성표체,단모형조적표체유지재교고수평,재각시기균명현고우동기가수술조(P<0.01).결과증실,거란소골질소송모형대서발병과정중,성골세포Cyclin D1、CDK4、p21단백출현명현고표체.제시성골세포증식가쾌,동시성골세포주기수조체역증다,성골세포수량상대불족,골형성저우골흡수,도치골질소송적발생.
BACKGROUND:G_1 phase regulatory protein plays an important role in regulating cell reproductive cycle.However,there are fewreports concerning research of G_1 phase regulatory protein of osteoblasts,and the relationship between postmenopausal osteoporosis and the G_1 phase regulatory protein of osteoblasts have not been explained.OBJECTIVE:To observe the change of G_1 phase regulatory protein of osteoblasts in the ovariectomized osteoporosis rats,and to investigate the pathogenesis of postmenopausal osteoporosis.METHODS:Totally 100 6-month-old SD female rats were randomly divided into sham surgery and model groups,with 50 animals in each group.Rats in the model group were performed bilaterally ovariectomy,those in the sham surgery group were subjected to sham surgery.Ten rats of each group were sacrificed at 4,8,12,18 and 24 weeks after operationt respectively.The expression of Cyclin D1,CDK4,and p21 protein in osteoblasts of lumbar vertebra were measured by lmmunohistochemistry.RESULTS AND CONCLUSION:Cyclin D1 and CDK4-positive expression mainly located at osteoblasts which in the superficies of bone trabecula.There was Cyclin D1 and CDK4 positive expression in the sham surglery group,but was obviously smaller than that of the model group.The location of p21 positive expression was similar to Cyclin D1.Obviously positive expression of p21 appeared in the bone tissue of both groups,in which that of the model group maintained at a high level,and higher than that of the sham surgery group at different periods after operation(P<0.01).The results demonstrated that Cyclin D1,CDK4 and p21 in osteoblasts were hyper-expressed in the process of osteoporosis in ovariectomized rats.It suggested that faster osteoblastproliferation and inhabited osteoblast cell cycles result in relative insufficiency of osteoblast,thus the bone formation is smaller than bone resorption,eventually,lead to the occurrence of osteoporosis.