中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2008年
8期
703-706
,共4页
单纯疱疹病毒%细胞周期蛋白依赖性激酶2%病毒复制
單純皰疹病毒%細胞週期蛋白依賴性激酶2%病毒複製
단순포진병독%세포주기단백의뢰성격매2%병독복제
Herpes simplex virus%Cyclindependent kinase 2%Virus replication
目的 探讨细胞周期蛋白依赖性激酶(CDK)2在单纯疱疹病毒(HSV)复制中的作用. 方法 以能够诱导表达CDK2显性负突变体致使CDK2功能缺陷的人结肠癌细胞株HT29Tet-Ondn-CDK2为材料,用免疫共沉淀及放射自显影法测定HSV感染后不同时间CDK2活性,并进行HSV的增殖动力学分析. 结果 HSV感染6h后CDK2活性被诱导,9h达到最大;一步生长曲线结果表明在CDK2活性被诱导前HSV处于静止状态,CDK2活性被诱导后HSV即进入快速的生长复制阶段. 结论 CDK2是HSV复制所必需的,HSV通过诱导宿主细胞CDK2活性而促进本身的复制.
目的 探討細胞週期蛋白依賴性激酶(CDK)2在單純皰疹病毒(HSV)複製中的作用. 方法 以能夠誘導錶達CDK2顯性負突變體緻使CDK2功能缺陷的人結腸癌細胞株HT29Tet-Ondn-CDK2為材料,用免疫共沉澱及放射自顯影法測定HSV感染後不同時間CDK2活性,併進行HSV的增殖動力學分析. 結果 HSV感染6h後CDK2活性被誘導,9h達到最大;一步生長麯線結果錶明在CDK2活性被誘導前HSV處于靜止狀態,CDK2活性被誘導後HSV即進入快速的生長複製階段. 結論 CDK2是HSV複製所必需的,HSV通過誘導宿主細胞CDK2活性而促進本身的複製.
목적 탐토세포주기단백의뢰성격매(CDK)2재단순포진병독(HSV)복제중적작용. 방법 이능구유도표체CDK2현성부돌변체치사CDK2공능결함적인결장암세포주HT29Tet-Ondn-CDK2위재료,용면역공침정급방사자현영법측정HSV감염후불동시간CDK2활성,병진행HSV적증식동역학분석. 결과 HSV감염6h후CDK2활성피유도,9h체도최대;일보생장곡선결과표명재CDK2활성피유도전HSV처우정지상태,CDK2활성피유도후HSV즉진입쾌속적생장복제계단. 결론 CDK2시HSV복제소필수적,HSV통과유도숙주세포CDK2활성이촉진본신적복제.
Objective To explore the effect of cyclindependent kinase(CDK)2 on the replication of herpes simplex virus(HSV).Methods Dominant-negative CDK2 expressing cell line HT29Tet-On dn-CDl(2 was used as host cells to determine the role of CDK2 in HSV rephcation.HT29Tet-0n dn-CDK2 cells were infected with HSV.The activity of CDK2 Was determined by the immunoprecipitate and autoradiograph before and after HSV infection.The growth curve of HSV Was conducted.Results The CDK2 activity was induced after infection for 6 h and reached the hishest level at 9 h.HSV replicated rapidly and productively after the CDK2 was induced.Coatiusion CDK2 is required for the replication of HSV,and HSV replicates in host cells by inducing the CDK2 activity.
