肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2011年
10期
674-677
,共4页
王忠明%刘桂荣%黄关宏%马建新%刘小林%陈丹丹%周婵%袁春銮%李秀翠
王忠明%劉桂榮%黃關宏%馬建新%劉小林%陳丹丹%週嬋%袁春鑾%李秀翠
왕충명%류계영%황관굉%마건신%류소림%진단단%주선%원춘란%리수취
食管肿瘤%T淋巴细胞亚群%杀伤细胞%天然%放射疗法
食管腫瘤%T淋巴細胞亞群%殺傷細胞%天然%放射療法
식관종류%T림파세포아군%살상세포%천연%방사요법
Esophageal neoplasms%T lymphocyte subsets%Killer cells,natural%Radiotherapy
目的 探讨行放疗的食管癌患者外周血T淋巴细胞、自然杀伤(NK)细胞的变化及其与放疗疗效的相关性.方法 对56例经病理组织学确诊的食管癌患者采用单纯放疗,应用流式细胞术测定其治疗前后外周血T淋巴细胞亚群及NK细胞比例,评价其与近、远期治疗效果的相关性.以30名健康体检者外周血为健康对照.结果 食管癌患者外周血T细胞比例、Th细胞比例、Th/Ts分别为( 58.3±5.2)％、(28.7±5.0)％、1.0±0.3,低于健康对照组的(65.8±7.2)％、(38.1±7.7)％、1.6±0.7(均P＜ 0.05)；而Ts细胞比例为(28.8±5.3)％,高于健康对照组的(25.4±5.7)％(P＜0.05).外周血T淋巴细胞亚群及Th/Ts的变化与患者性别、肿瘤分期、病理分化程度、病变部位等临床病理参数之间的差异均无统计学意义(均P＞ 0.05).放疗后,患者外周血总T细胞比例、Th细胞比例、Th/Ts及NK细胞比例分别为( 66.9±4.5)％、(40.6±5.6)％、(1.6±0.5)％和(16.2±3.9)％,均高于治疗前的(59.4±4.9)％、( 29.1±4.2)％、(1.0±0.4)％和(14.6±3.2)％(均P＜ 0.05),而Ts细胞比例治疗后为(25.4±3.6)％,较治疗前的( 28.4±5.7)％降低(P＜0.05).总T细胞、Th细胞增高程度与病变进展密切相关,差异有统计学意义(均P＜ 0.05).结论 T淋巴细胞亚群、NK细胞可用于行放疗的食管癌患者的免疫监测.检测患者外周血相关指标的表达对评估患者的免疫功能、治疗及预后具有一定的临床意义.
目的 探討行放療的食管癌患者外週血T淋巴細胞、自然殺傷(NK)細胞的變化及其與放療療效的相關性.方法 對56例經病理組織學確診的食管癌患者採用單純放療,應用流式細胞術測定其治療前後外週血T淋巴細胞亞群及NK細胞比例,評價其與近、遠期治療效果的相關性.以30名健康體檢者外週血為健康對照.結果 食管癌患者外週血T細胞比例、Th細胞比例、Th/Ts分彆為( 58.3±5.2)％、(28.7±5.0)％、1.0±0.3,低于健康對照組的(65.8±7.2)％、(38.1±7.7)％、1.6±0.7(均P＜ 0.05)；而Ts細胞比例為(28.8±5.3)％,高于健康對照組的(25.4±5.7)％(P＜0.05).外週血T淋巴細胞亞群及Th/Ts的變化與患者性彆、腫瘤分期、病理分化程度、病變部位等臨床病理參數之間的差異均無統計學意義(均P＞ 0.05).放療後,患者外週血總T細胞比例、Th細胞比例、Th/Ts及NK細胞比例分彆為( 66.9±4.5)％、(40.6±5.6)％、(1.6±0.5)％和(16.2±3.9)％,均高于治療前的(59.4±4.9)％、( 29.1±4.2)％、(1.0±0.4)％和(14.6±3.2)％(均P＜ 0.05),而Ts細胞比例治療後為(25.4±3.6)％,較治療前的( 28.4±5.7)％降低(P＜0.05).總T細胞、Th細胞增高程度與病變進展密切相關,差異有統計學意義(均P＜ 0.05).結論 T淋巴細胞亞群、NK細胞可用于行放療的食管癌患者的免疫鑑測.檢測患者外週血相關指標的錶達對評估患者的免疫功能、治療及預後具有一定的臨床意義.
목적 탐토행방료적식관암환자외주혈T림파세포、자연살상(NK)세포적변화급기여방료료효적상관성.방법 대56례경병리조직학학진적식관암환자채용단순방료,응용류식세포술측정기치료전후외주혈T림파세포아군급NK세포비례,평개기여근、원기치료효과적상관성.이30명건강체검자외주혈위건강대조.결과 식관암환자외주혈T세포비례、Th세포비례、Th/Ts분별위( 58.3±5.2)％、(28.7±5.0)％、1.0±0.3,저우건강대조조적(65.8±7.2)％、(38.1±7.7)％、1.6±0.7(균P＜ 0.05)；이Ts세포비례위(28.8±5.3)％,고우건강대조조적(25.4±5.7)％(P＜0.05).외주혈T림파세포아군급Th/Ts적변화여환자성별、종류분기、병리분화정도、병변부위등림상병리삼수지간적차이균무통계학의의(균P＞ 0.05).방료후,환자외주혈총T세포비례、Th세포비례、Th/Ts급NK세포비례분별위( 66.9±4.5)％、(40.6±5.6)％、(1.6±0.5)％화(16.2±3.9)％,균고우치료전적(59.4±4.9)％、( 29.1±4.2)％、(1.0±0.4)％화(14.6±3.2)％(균P＜ 0.05),이Ts세포비례치료후위(25.4±3.6)％,교치료전적( 28.4±5.7)％강저(P＜0.05).총T세포、Th세포증고정도여병변진전밀절상관,차이유통계학의의(균P＜ 0.05).결론 T림파세포아군、NK세포가용우행방료적식관암환자적면역감측.검측환자외주혈상관지표적표체대평고환자적면역공능、치료급예후구유일정적림상의의.
Objective To study the relationship between the change of T lymphocyte subsets and NK cells in esophageal cancer patients and radiotherapy effect.Methods The levels of T lymphocyte subsets and NK cells were detected by flow cytometry in 56 cases with histologically confirmed esophageal cancer treated with radiotherapy and contrasted to the healthy people.Results In patients with esophageal cancer,peripheral blood T cells,Th cells,Th / Ts were decreased significantly compared with the control group [(58.3±5.2) ％ vs (65.8±7.2) ％,(28.7±5.0) ％ vs (38.1±7.7) ％,(1.0±0.3) vs (1.6+2.7),all P ＜ 0.05],while the Ts cells were significantly increased [(28.8±5.3) ％ vs (25.4±5.7) ％,P ＜ 0.05].There was no significant difference between peripheral blood T lymphocyte subsets,Th/Ts ratio change and patient age,sex,tumor staging,histological differentiation and pathological lesions.After radiotherapy,the levels of peripheral blood T cells,Th cells,Th/Ts cell ratio and NK cells in esophageal cancer patients were increased [(66.9±4.5) ％ vs (59.4±4.9) ％,(40.6±5.6) ％ vs (29.1±4.2) ％,(1.6+0.5) ％ vs (1.0±0.4) ％,(16.2±3.9) ％ vs (14.6±3.2) ％,all P ＜ 0.05],while the Ts cells decreased [ (25.4±3.6) ％ vs (28.4±5.7) ％,P ＜ 0.05].The increasing degree of peripheral blood T cells,Th cells were closely related to the lesion progress,the difference was significant (both P ＜ 0.05).Conclusion Cellular immune function in patients with esophageal cancer is low.Detection of T lymphocyte subsets,NK cells can be used for immune monitoring of patients with esophageal cancer.
