CAJ | 학술논문

目的探讨原发性高血压患者外周血单核细胞的端粒酶活性与p53抑癌基因突变的相关性.方法用端粒酶TRAP-ELISA方法测定75例原发性高血压患者(高血压组)和50例健康者(对照组)外周血单核细胞端粒酶活性,同时用PCR-SSCP测定所有标本的p53抑癌基因第5～8号外显子的突变情况.结果高血压级端料酶阳性率50.7%(38/75),与对照组26.0%(13/50)相比,差异有统计学意义(P＜0.05);与端粒酶活性有关的主要因素为年龄及收缩期血压,优势比分别为1.0418和1.0468.仅有2例高血压患者发生p53抑癌基因的突变.结论端粒酶是细胞增殖活性的分子水平标记物,它的激活可能是原发性高血压的发病因素之一并能促进病变的发展.高血压病与p53抑癌基因突变关系不明显,但该基因可能激活端粒酶活性.
목적 탐토원발성고혈압환자외주혈단핵세포적단립매활성여p53억암기인돌변적상관성.방법 용단립매TRAP-ELISA방법측정75례원발성고혈압환자(고혈압조)화50례건강자(대조조)외주혈단핵세포단립매활성,동시용PCR-SSCP측정소유표본적p53억암기인제5～8호외현자적돌변정황.결과 고혈압급단료매양성솔50.7%(38/75),여대조조26.0%(13/50)상비,차이유통계학의의(P＜0.05);여단립매활성유관적주요인소위년령급수축기혈압,우세비분별위1.0418화1.0468.부유2례고혈압환자발생p53억암기인적돌변.결론 단립매시세포증식활성적분자수평표기물,타적격활가능시원발성고혈압적발병인소지일병능촉진병변적발전.고혈압병여p53억암기인돌변관계불명현,단해기인가능격활단립매활성.
Objective To investigate the telomerase activity of peripheral blood mononuclear cells in patients with essential hypertension,and to detect the mutation of p53 tumor suppressor gene.Methods The telomerase activity of peripheral blood mononuclear cells in 75 hypertensive and 50 normotensive was measured by telomerase-PCR-ELISA assay.The mutation of p53 tumor suppressor was detected by PCR-SSCP.Results The telomerase activity of peripheral blood mononuelear cells in hypertensive group were significantly increased than those in control group.Logistic regression study showed that the main influenced factors were age,diastolic blood pressure and the mutation of p53 tumor suppressor.The mutation of p53 tumor suppressor gene may increase the telomerase activity.The telomerase activity was correlated with the severity of hypertension and may be a prognostic risk factor.The increased telomerase activity may be related to the target organ injure of essential hypertension.Conclusion The telomerase activity was a mark of cellular proliferation activity in molecular level.It may be an important factor in the pathogenesis of essential hypertension.Meanwhile,the up-regulation of telomerase activity may be one of the risk factors in essential hypertension.