CAJ | 학술논문

目的探讨BIOMED-2聚合酶链反应(PCR)在成熟非霍奇金B细胞淋巴瘤(B-NHL)诊断中的价值.方法收集成熟B-NHL组织标本72例,其中弥漫性大B细胞淋巴瘤37例,黏膜相关淋巴组织结外边缘区淋巴瘤35例为研究对象,并以反应性增生病变25例作为对照.提取以上组织的DNA,并以PCR来检测其完整性和可扩增性,选取质量合格的DNA.85.6%(83/97)的样品DNA长度>300 bp,其中60例成熟B-NHL和23例反应性增生可用于BIOMED-2 PCR检测免疫球蛋白重链(IgH)和kappa轻链(IgK)基因重排的克隆性.结果利用BIOMED-2 PCR检测的60例成熟B-NHL中,57例存在Ig基因的克隆性重排,其检测敏感性为95%,23例反应性增生病例中未出现Ig基因的克隆性重排,其检测特异性为100%.结论 BIOMED-2 PCR适用于石蜡包埋组织.该方法具有很高的敏感性和特异性,对成熟B-NHL诊断的辅助价值很高.
목적 탐토BIOMED-2취합매련반응(PCR)재성숙비곽기금B세포림파류(B-NHL)진단중적개치.방법 수집성숙B-NHL조직표본72례,기중미만성대B세포림파류37례,점막상관림파조직결외변연구림파류35례위연구대상,병이반응성증생병변25례작위대조.제취이상조직적DNA,병이PCR래검측기완정성화가확증성,선취질량합격적DNA.85.6%(83/97)적양품DNA장도>300 bp,기중60례성숙B-NHL화23례반응성증생가용우BIOMED-2 PCR검측면역구단백중련(IgH)화kappa경련(IgK)기인중배적극륭성.결과 이용BIOMED-2 PCR검측적60례성숙B-NHL중,57례존재Ig기인적극륭성중배,기검측민감성위95%,23례반응성증생병례중미출현Ig기인적극륭성중배,기검측특이성위100%.결론 BIOMED-2 PCR괄용우석사포매조직.해방법구유흔고적민감성화특이성,대성숙B-NHL진단적보조개치흔고.
Objective To evaluate the efficiency of the BIOMED-2 PCR assay and its implication in the diagnosis of mature B-cell non-Hodgkin's lymphomas. Methods Clinical, morphological and immunohistochemical features of 72 cases of non-Hodgkin's lymphomas were studied, including 25 reactive lymphoid hyperplasia, 37 diffuse large B cell lymphomas (DLBCL) and 35 extranodal marginal zone lymphomas of mucosa associated lymphoid tissues (MALT lymphoma and in addition, 25 cases of reactive lymphoid hyperplasia were used as the controls). DNA was exacted from the paraffin embedded formalin fixed tissue blocks and the quality of DNA was assessed using the BIOMED-2 specimen control reaction. Adequate samples were then analyzed by BIOMED-2 for immunoglobulin heavy and kappa light chain rearrangements. Results Adequate DNA was obtained in 83 of 97 samples, including 60 mature B cell lymphomas and 23 reactive lymphoid hyperplasia. Clonal B-cell gene rearrangements were detected in 57 of 60(95%) lymphomas. In contrast, clonal Ig gene rearrangements were not detected in any of the 23 cases of reactive lymphoid hyperplasia. Conclusion BIOMED-2 assay is highly sensitive and specific for the detection of clonal B cell gene rearrangement using routine paraffin embedded formalin fixed specimens.