白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2011年
7期
389-391,394
,共4页
肿瘤,实验性%白藜芦醇%Molt-4细胞%细胞凋亡%WAVE1基因
腫瘤,實驗性%白藜蘆醇%Molt-4細胞%細胞凋亡%WAVE1基因
종류,실험성%백려호순%Molt-4세포%세포조망%WAVE1기인
Neoplasms,experimental%Resveratrol%Molt-4 cells%Apoptosis%WAVE1
目的 探讨白藜芦醇诱导人类急性淋巴细胞白血病Molt-4细胞凋亡的作用机制.方法 噻唑蓝比色法(MTF)测定细胞生长抑制率;流式细胞术检测细胞周期分布及凋亡率;半定量反转录聚合酶链反应(RT-PCR)法检测WAVE1基因的表达.结果 MTT结果显示:12.5、25.0、50.0、100.0、200.0 μmol/L的白藜芦醇作用于Moh-4细胞24、48、72 h后,细胞的最大抑制率分别达到29.32%、36.11%、53.92%、62.50%、74.98%,并呈时间-剂量依赖性(F=33.614,P<0.05);流式细胞术检测结果显示:与对照组相比,50.0、100.0 μmol/L白藜芦醇作用于Molt-4细胞48 h后,S期细胞比例由42.2%分别上升为68.6%和78.1%,细胞发生了S期阻滞(F=19.453,P<0.01);PCR结果显示:50.0、100.0 μmol/L的白藜芦醇处理Molt-4细胞48 h后,WAVE1/GAPDH比值分别为0.356±0.03、0.382±0.05,与对照组(0.586±0.06)比较,差异有统计学意义(F=8.950,P<0.01).结论 白藜芦醇可诱导Moh-4细胞发生凋亡,其作用机制可能与下调WAVE1基因表达有关.
目的 探討白藜蘆醇誘導人類急性淋巴細胞白血病Molt-4細胞凋亡的作用機製.方法 噻唑藍比色法(MTF)測定細胞生長抑製率;流式細胞術檢測細胞週期分佈及凋亡率;半定量反轉錄聚閤酶鏈反應(RT-PCR)法檢測WAVE1基因的錶達.結果 MTT結果顯示:12.5、25.0、50.0、100.0、200.0 μmol/L的白藜蘆醇作用于Moh-4細胞24、48、72 h後,細胞的最大抑製率分彆達到29.32%、36.11%、53.92%、62.50%、74.98%,併呈時間-劑量依賴性(F=33.614,P<0.05);流式細胞術檢測結果顯示:與對照組相比,50.0、100.0 μmol/L白藜蘆醇作用于Molt-4細胞48 h後,S期細胞比例由42.2%分彆上升為68.6%和78.1%,細胞髮生瞭S期阻滯(F=19.453,P<0.01);PCR結果顯示:50.0、100.0 μmol/L的白藜蘆醇處理Molt-4細胞48 h後,WAVE1/GAPDH比值分彆為0.356±0.03、0.382±0.05,與對照組(0.586±0.06)比較,差異有統計學意義(F=8.950,P<0.01).結論 白藜蘆醇可誘導Moh-4細胞髮生凋亡,其作用機製可能與下調WAVE1基因錶達有關.
목적 탐토백려호순유도인류급성림파세포백혈병Molt-4세포조망적작용궤제.방법 새서람비색법(MTF)측정세포생장억제솔;류식세포술검측세포주기분포급조망솔;반정량반전록취합매련반응(RT-PCR)법검측WAVE1기인적표체.결과 MTT결과현시:12.5、25.0、50.0、100.0、200.0 μmol/L적백려호순작용우Moh-4세포24、48、72 h후,세포적최대억제솔분별체도29.32%、36.11%、53.92%、62.50%、74.98%,병정시간-제량의뢰성(F=33.614,P<0.05);류식세포술검측결과현시:여대조조상비,50.0、100.0 μmol/L백려호순작용우Molt-4세포48 h후,S기세포비례유42.2%분별상승위68.6%화78.1%,세포발생료S기조체(F=19.453,P<0.01);PCR결과현시:50.0、100.0 μmol/L적백려호순처리Molt-4세포48 h후,WAVE1/GAPDH비치분별위0.356±0.03、0.382±0.05,여대조조(0.586±0.06)비교,차이유통계학의의(F=8.950,P<0.01).결론 백려호순가유도Moh-4세포발생조망,기작용궤제가능여하조WAVE1기인표체유관.
Objective To investigate the possible mechanism of apoptosis effect induced by resveratrol in human acute T lymphoblast leukemia Molt-4 cells. Methods MTT method was used to analyze the inhibition rate. The cell cycle and apoptosis percentage of Molt-4 cells treated with resveratrol were detected by flow cytometry. The expression of WAVE1 mRNA was assessed by semi-quantitative RT-PCR. Results After treated with 12.5, 25.0, 50.0, 100.0, 200.0μmol/L resveratrol for 24, 48 and 72 hours, the inhibitory effects were at 29.32 %, 36.11 %, 53.92 %, 62.50 %, and 74.98 %, respectively. Resveratrol was able to inhibit the proliferation of Molt-4 cells in a time-dependent and dose-dependent manner (F =33.614, P <0.05).Compared with control group, after treated with 50.0, 100.0 μmol/L resveratrol for 48 h, the cell number in S phase of Molt-4 cells was 68.6 % and 78.1 %, respectively, and the cell cycle was arrested at S phase (F = 19.453, P < 0.01) after treated with 50.0, 100.0 μmol/L resveratrol for 48 h, the ratio of WAVE1/GAPDH was 0.356±0.03, 0.382±0.05. Compared with control group 0.586±0.06, the ratio of WAVE1/GAPDH was decreased (F =8.950, P <0.01). Conclusion Resveratrol could induce the cells to undergo apoptosis, which was probably related to downregulation the expression of WAVE1.
