中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2009年
7期
459-462
,共4页
钳志晶%张荣富%马丽%吴东海
鉗誌晶%張榮富%馬麗%吳東海
겸지정%장영부%마려%오동해
红斑狼疮,系统性%狼疮肾炎%补体1q%补体3%补体4
紅斑狼瘡,繫統性%狼瘡腎炎%補體1q%補體3%補體4
홍반랑창,계통성%랑창신염%보체1q%보체3%보체4
Lupus erythematosus,systemic%Lupus nephritis%Complement lq%Complement 3%Complement 4
目的 探讨末端补体SC5b-9、抗C1q抗体及补体C3和C4在狼疮疾病活动性判定中的价值.方法 采用酶联免疫吸附试验(ELISA)检测系统性红斑狼疮(SLE)组62例、疾病对照组35例和健康对照组35名血清中的末端补体SC5b-9和抗C1q抗体水平,并检测血清中补体C3和C4的水平,并与SLE患者临床表现、病情活动度及狼疮肾炎(LN)的病理分型进行相关性分析.结果 在SLE活动组中末端补体SC5b-9和抗C1q抗体水平均显著高于各对照组(均P<0.05).末端补体SC5b-9和抗C1q抗体与补体C3、C4呈负相关(P<0.05),与SLE疾病活动指数(SLEDAI)呈正相关(P<0.05).联合检测对疾病活动的判断的敏感性和特异性分别为95%和98%.末端补体SC5b-9及抗C1q抗体在狼疮增殖件肾炎中表达明显高于膜性肾炎(均P<0.05).结论 联合检测可提高疾病诊断和活动性判断的特异性和敏感性;末端补体SC5b-9和抗C1q抗体参与了狼疮增殖性肾炎的免疫发病机制.
目的 探討末耑補體SC5b-9、抗C1q抗體及補體C3和C4在狼瘡疾病活動性判定中的價值.方法 採用酶聯免疫吸附試驗(ELISA)檢測繫統性紅斑狼瘡(SLE)組62例、疾病對照組35例和健康對照組35名血清中的末耑補體SC5b-9和抗C1q抗體水平,併檢測血清中補體C3和C4的水平,併與SLE患者臨床錶現、病情活動度及狼瘡腎炎(LN)的病理分型進行相關性分析.結果 在SLE活動組中末耑補體SC5b-9和抗C1q抗體水平均顯著高于各對照組(均P<0.05).末耑補體SC5b-9和抗C1q抗體與補體C3、C4呈負相關(P<0.05),與SLE疾病活動指數(SLEDAI)呈正相關(P<0.05).聯閤檢測對疾病活動的判斷的敏感性和特異性分彆為95%和98%.末耑補體SC5b-9及抗C1q抗體在狼瘡增殖件腎炎中錶達明顯高于膜性腎炎(均P<0.05).結論 聯閤檢測可提高疾病診斷和活動性判斷的特異性和敏感性;末耑補體SC5b-9和抗C1q抗體參與瞭狼瘡增殖性腎炎的免疫髮病機製.
목적 탐토말단보체SC5b-9、항C1q항체급보체C3화C4재랑창질병활동성판정중적개치.방법 채용매련면역흡부시험(ELISA)검측계통성홍반랑창(SLE)조62례、질병대조조35례화건강대조조35명혈청중적말단보체SC5b-9화항C1q항체수평,병검측혈청중보체C3화C4적수평,병여SLE환자림상표현、병정활동도급랑창신염(LN)적병리분형진행상관성분석.결과 재SLE활동조중말단보체SC5b-9화항C1q항체수평균현저고우각대조조(균P<0.05).말단보체SC5b-9화항C1q항체여보체C3、C4정부상관(P<0.05),여SLE질병활동지수(SLEDAI)정정상관(P<0.05).연합검측대질병활동적판단적민감성화특이성분별위95%화98%.말단보체SC5b-9급항C1q항체재랑창증식건신염중표체명현고우막성신염(균P<0.05).결론 연합검측가제고질병진단화활동성판단적특이성화민감성;말단보체SC5b-9화항C1q항체삼여료랑창증식성신염적면역발병궤제.
Objective To explore the value of serum SC5b-9, anti-C1q antibody, C3 and C4 levels in the assessment of lupus activity. Methods The enzyme linked immunosorbent assay (ELISA) was used to measure SC5b-9 and anti-C1q antibody, rate nepheiometry was used to detect the serum level of C3 and C4 in sera of 62 SLE patients, 35 patients with other rheumatic diseases (including rheumatoid arthritis, ankylosing spondylitis, primary Sjogren' s syndrome, mixed connective tissue disease, dermatomyositis, polymyositis, systemic sclerosis and vasculitis) and 35 healthy controls. And the correlation between above-mentioned parameters and lupus clinical manifestations, disease activity and histological type of lupus nephritis were analyzed. Results In SLE patients, the levels of SC5b-9 and anti-C1q antibody were significantly higher than those in patients with other rheumatic diseases and healthy controls (P<0.05). The titers of SC5b-9 and anti-C1q antibody negatively correlated with C3 and C4 (P<0.05), and positively correlated with SLEDAI (P<0.05). The sensitivity and specificity of the combination of these three measurements for SLE was 95.37% and 98.46 respectively. SC5b-9 and anti-C1q antibody were associated with the presence of proliferative glomerulonephritis (P <0.05). Conclusion Taking the evaluation of all these three measurements simultaneously is valuable for the diagnosis of lupus flare. SC5b-9 and anti-C1q antibody may play major roles in the immunopathogenesis of lupus nephritis.
