CAJ | 학술논문

目的观察邻苯二酚类螯合剂9501、9502、7601(CBMIDA)对辐射引起大鼠肝线粒体损伤的保护作用，以探讨其防护核素内污染辐射损伤的可能性。方法采用γ射线外照射方法，引起大鼠肝线粒体辐射损伤，给予不同剂量的螯合剂，观察其保护作用。以硫代巴比妥酸比色法测定脂质过氧化产物MDA含量；在520 nm波长测定反应体系的浊度变化，以反映线粒体肿胀损伤程度，并制备电镜切片。结果 9501(5×10-6 mol/L)、9502(10-5 mol/L)、7601(10-5 mol/L)能显著抑制120 Gy γ辐射所致肝线粒体MDA生成，9501、7601对辐射引起的线粒体肿胀也有明显的保护作用，9501并能防止辐射所致线粒体形态的病理性损伤。作者并对此类螯合剂对大鼠肝线粒体辐射损伤的保护作用机理进行了初步探讨。结论邻苯二酚类螯合剂9501、9502、7601(CBMIDA)对外照射引起大鼠肝线粒体损伤有明显的保护作用。
목적관찰린분이분류오합제9501、9502、7601(CBMIDA)대복사인기대서간선립체손상적보호작용，이탐토기방호핵소내오염복사손상적가능성。방법채용γ사선외조사방법，인기대서간선립체복사손상，급여불동제량적오합제，관찰기보호작용。이류대파비타산비색법측정지질과양화산물MDA함량；재520 nm파장측정반응체계적탁도변화，이반영선립체종창손상정도，병제비전경절편。결과 9501(5×10-6 mol/L)、9502(10-5 mol/L)、7601(10-5 mol/L)능현저억제120 Gy γ복사소치간선립체MDA생성，9501、7601대복사인기적선립체종창야유명현적보호작용，9501병능방지복사소치선립체형태적병이성손상。작자병대차류오합제대대서간선립체복사손상적보호작용궤리진행료초보탐토。결론린분이분류오합제9501、9502、7601(CBMIDA)대외조사인기대서간선립체손상유명현적보호작용。
Objective To evaluate the effects of catecholomic acid derivatives 9501,9502 and 7601 (CBMIDA) against radiation-induced injury of rat liver mitochondria in vitro. Methods The injury of rat liver mitochondria was induced by γ-irradiation in vitro.The contents of MDA were assayed by spectrophotometry of TBA.The absorption value at 520 nm was measured to detect swelling of mitochondria.The electron microscopic samples of mitochondria were prepared. Results All 9501 (5×10-6 mol/L),9502(10-5 mol/L),and 7601(10-5 mol/L) significantly inhibited radiation-induced increase of MDA information.The swelling of mitochondria induced by irradiation was also prevented by 9501,7601.The electron micrographs also showed that 9501 markedly reduced the pathological damage of mitochondria induced by γ-irradiation.The mechanisms of antioxidative action of catecholomic acid derivatives was discussed. Conclusion Injurious effect of radiation on rat liver mitochondria can be prevented by catecholomic acid derivatives 9501,9502 and 7601 (CBMIDA).