中华医学杂志(英文版)
中華醫學雜誌(英文版)
중화의학잡지(영문판)
CHINESE MEDICAL JOURNAL
2002年
10期
1576-1578
,共3页
TCM%治疗%包虫病%泡球蚴病
TCM%治療%包蟲病%泡毬蚴病
TCM%치료%포충병%포구유병
treatment%alveolar echinococcosis%Tradit ional Chinese Medicine
目的评估一种新的TCM提取化合物治疗小鼠泡型包虫病的效果.方法准备二个先决条件,第一是从TCM中提取一种化合物,要通过一系列程序包括中药混合,置平底烧瓶内加热煮沸,用冷凝回流装置和微火蒸发,最后形成为深棕色粉剂.第二是建立泡球蚴病动物模型,从感染泡球蚴病的小鼠获取小片泡球蚴组织,移植接种50只昆明小鼠腹腔内,接种后受染期分为1周感染和10周感染,二者再分为治疗组和对照组.泡球蚴抑制率测定和电镜检查作为判定疗效的主要方法.治疗组感染鼠每天灌胃TCM提取化合物一次,剂量2 0 mg/kg,连用3个月.TCM治毕半月,处死剖检所有动物.结果 1周感染后治疗组9只鼠的泡球蚴总湿重37.76 g(±s, 4.196±2.090 g),明显低于10只对照鼠(121.294 g, ±s, 12.129±4.305 g).泡球蚴抑制率为65.7%(P< 0.01).同样地,10周感染后治疗组7只鼠的泡球蚴总湿重4.326 g( ±s, 0.619±1.207 g),明显低于6只对照鼠(17.857 g, ±s , 2.926±3.275 g).泡球蚴抑制率为80.6%(P<0.01).泡球蚴超微结构在治疗组与对照组之间显示明显的区别.结论 TCM提取粉治疗小鼠泡球蚴病,是一种有希望的抗包虫化合物,进一步研究有意义.
目的評估一種新的TCM提取化閤物治療小鼠泡型包蟲病的效果.方法準備二箇先決條件,第一是從TCM中提取一種化閤物,要通過一繫列程序包括中藥混閤,置平底燒瓶內加熱煮沸,用冷凝迴流裝置和微火蒸髮,最後形成為深棕色粉劑.第二是建立泡毬蚴病動物模型,從感染泡毬蚴病的小鼠穫取小片泡毬蚴組織,移植接種50隻昆明小鼠腹腔內,接種後受染期分為1週感染和10週感染,二者再分為治療組和對照組.泡毬蚴抑製率測定和電鏡檢查作為判定療效的主要方法.治療組感染鼠每天灌胃TCM提取化閤物一次,劑量2 0 mg/kg,連用3箇月.TCM治畢半月,處死剖檢所有動物.結果 1週感染後治療組9隻鼠的泡毬蚴總濕重37.76 g(±s, 4.196±2.090 g),明顯低于10隻對照鼠(121.294 g, ±s, 12.129±4.305 g).泡毬蚴抑製率為65.7%(P< 0.01).同樣地,10週感染後治療組7隻鼠的泡毬蚴總濕重4.326 g( ±s, 0.619±1.207 g),明顯低于6隻對照鼠(17.857 g, ±s , 2.926±3.275 g).泡毬蚴抑製率為80.6%(P<0.01).泡毬蚴超微結構在治療組與對照組之間顯示明顯的區彆.結論 TCM提取粉治療小鼠泡毬蚴病,是一種有希望的抗包蟲化閤物,進一步研究有意義.
목적평고일충신적TCM제취화합물치료소서포형포충병적효과.방법준비이개선결조건,제일시종TCM중제취일충화합물,요통과일계렬정서포괄중약혼합,치평저소병내가열자비,용냉응회류장치화미화증발,최후형성위심종색분제.제이시건립포구유병동물모형,종감염포구유병적소서획취소편포구유조직,이식접충50지곤명소서복강내,접충후수염기분위1주감염화10주감염,이자재분위치료조화대조조.포구유억제솔측정화전경검사작위판정료효적주요방법.치료조감염서매천관위TCM제취화합물일차,제량2 0 mg/kg,련용3개월.TCM치필반월,처사부검소유동물.결과 1주감염후치료조9지서적포구유총습중37.76 g(±s, 4.196±2.090 g),명현저우10지대조서(121.294 g, ±s, 12.129±4.305 g).포구유억제솔위65.7%(P< 0.01).동양지,10주감염후치료조7지서적포구유총습중4.326 g( ±s, 0.619±1.207 g),명현저우6지대조서(17.857 g, ±s , 2.926±3.275 g).포구유억제솔위80.6%(P<0.01).포구유초미결구재치료조여대조조지간현시명현적구별.결론 TCM제취분치료소서포구유병,시일충유희망적항포충화합물,진일보연구유의의.
Objective To evaluate the efficacy of a novel compound derived from Traditional Chinese Me dicine (TCM) for treatment of alveolar echinococcosis in the Kuming mouse.Results Total wet weight of alveococcus collected from the 9 mice in the treated group after one week of infection was 37.8 g (±s, 4.196±2.090 g), which was markedly lower than the amount collected from 10 mice of the control group (121.3 g, ±s, 12.1±4.3 g). Therefore, the inhibitory rate of th e alveococcus was 65.7% (P<0.01 ). Similarly, the total wet weight of alv eococcus from 7 mice of the treated group after 10 weeks of infection was 4.3 g (±s, 0.62±1.21 g), which was much lower than the amount collecte d from 6 mice of the control group (17.85 g, ±s, 2.93±3.28 g). The inhibitory rate of alveococcus was 80.6% (P<0.01). Furthermore, the u ltrastructural appearances of alveococcus showed obvious differences between the two groups.Conclusion TCM extractive powder for treatment of murine alveococcosis is considered to be a hopeful anti-echinococcus compound it is a topic for further study.
