农业科学与技术：英文版
農業科學與技術：英文版
농업과학여기술：영문판
Agricultural Science ＆ Technology
2011年
12期
1784-1786,1974
,共4页
邢佳毅%雷慧%程继鸿%杨瑞%王绍辉
邢佳毅%雷慧%程繼鴻%楊瑞%王紹輝
형가의%뢰혜%정계홍%양서%왕소휘
番茄根系%蛋白组学%SDS-PAGE
番茄根繫%蛋白組學%SDS-PAGE
번가근계%단백조학%SDS-PAGE
Tomato roots%Proteome%SDS-PAGE
[目的]建立一套适合番茄幼苗根系蛋白质组分析的SDS-PAGE方法,获得清晰的电泳图谱。[方法]以番茄幼苗根系为试材,对根系蛋白的提取方法和上样量等因素进行了优化。[结果]上样量为60μg时,改良后的TCA-丙酮沉淀法提取的蛋白能够获得分辨率较高、图像清晰、重复性好的SDS-PAGE图谱。[结论]该法可以基本满足番茄幼苗根系蛋白质组学的研究。
[目的]建立一套適閤番茄幼苗根繫蛋白質組分析的SDS-PAGE方法,穫得清晰的電泳圖譜。[方法]以番茄幼苗根繫為試材,對根繫蛋白的提取方法和上樣量等因素進行瞭優化。[結果]上樣量為60μg時,改良後的TCA-丙酮沉澱法提取的蛋白能夠穫得分辨率較高、圖像清晰、重複性好的SDS-PAGE圖譜。[結論]該法可以基本滿足番茄幼苗根繫蛋白質組學的研究。
[목적]건립일투괄합번가유묘근계단백질조분석적SDS-PAGE방법,획득청석적전영도보。[방법]이번가유묘근계위시재,대근계단백적제취방법화상양량등인소진행료우화。[결과]상양량위60μg시,개량후적TCA-병동침정법제취적단백능구획득분변솔교고、도상청석、중복성호적SDS-PAGE도보。[결론]해법가이기본만족번가유묘근계단백질조학적연구。
[Objective] This study aimed to develop an efficient SDS-PAGE method suitable for analyzing the total protein of Solanum lycopersicum seedling roots.[Method] By using the Solanum lycopersicum seedling roots as the experimental material,the protein extraction,sample volume and other factors influencing the final electrophoresis pattern were respectively optimized.[Result] The high-resolution,clear and reproducible SDS-PAGE pattern could be obtained when the protein was extracted with modified TCA-acetone precipitation and the sample volume for electrophoresis was 60 μg.[Conclusion] This optimized method could basically meet the requirements for study the proteome of tomato seedling roots.
