农业科学与技术:英文版
農業科學與技術:英文版
농업과학여기술:영문판
Agricultural Science & Technology
2012年
1期
32-35
,共4页
毕晓欣%黄玲%靖美东%韩立亚%郝月
畢曉訢%黃玲%靖美東%韓立亞%郝月
필효흔%황령%정미동%한립아%학월
菲律宾蛤仔%CO%I基因%遗传多样性%遗传分化
菲律賓蛤仔%CO%I基因%遺傳多樣性%遺傳分化
비률빈합자%CO%I기인%유전다양성%유전분화
V. philippinarum%CO I gene%Genetic diversity%VGenetic differentiation
[目的]了解我国沿海野生菲律宾蛤仔群体遗传多样性和遗传分化情况。[方法]采用PCR技术对烟台威海2个群体的线粒体cDI基因片段进行扩增和序列分析。[结果]50个样本均得到684bp的序列,共定义了42个单倍型,检测到62个变异位点,2个群体单倍型多样性都较高,而核苷酸多样性相对偏低。用最大似然法(MaximumLikelihood,ML)构建的单倍型系统进化树显示2个地理种群的样本呈现散在分布,没有明显的种群分化。[结论】反映了海洋动物的基因交流较容易,不同海域隔离较弱。
[目的]瞭解我國沿海野生菲律賓蛤仔群體遺傳多樣性和遺傳分化情況。[方法]採用PCR技術對煙檯威海2箇群體的線粒體cDI基因片段進行擴增和序列分析。[結果]50箇樣本均得到684bp的序列,共定義瞭42箇單倍型,檢測到62箇變異位點,2箇群體單倍型多樣性都較高,而覈苷痠多樣性相對偏低。用最大似然法(MaximumLikelihood,ML)構建的單倍型繫統進化樹顯示2箇地理種群的樣本呈現散在分佈,沒有明顯的種群分化。[結論】反映瞭海洋動物的基因交流較容易,不同海域隔離較弱。
[목적]료해아국연해야생비률빈합자군체유전다양성화유전분화정황。[방법]채용PCR기술대연태위해2개군체적선립체cDI기인편단진행확증화서렬분석。[결과]50개양본균득도684bp적서렬,공정의료42개단배형,검측도62개변이위점,2개군체단배형다양성도교고,이핵감산다양성상대편저。용최대사연법(MaximumLikelihood,ML)구건적단배형계통진화수현시2개지리충군적양본정현산재분포,몰유명현적충군분화。[결론】반영료해양동물적기인교류교용역,불동해역격리교약。
[Objective] The aim was to understand genetic diversity and genetic differ- entiation situation of wild V. philippinarum population in the coast of China. [Methed] Based on PCR technology, CO I gene fragments of the mitochondria were amplified from two populations of Yantai and Weihai for sequence analysis. [Result] Fifty sam- ples all obtained CO I gene fragment sequence of 684 bp. A total of 42 haplotypes were defined, and 62 variation loci were detected. The haplotypes diversity of two populations was both high, while the nucleotide diversity was low. The phylogenetic tree of haplotypes established by maximum likelihood (ML) method showed that the samples of two geographic populations presented diffused distribution and had no obvious population differentiation. [Conclusion] The result indicated that the gene ex- change of marine animal is easy, and the separation among different seas is weak.