广东化工
廣東化工
엄동화공
GUANGDONG CHEMICAL INDUSTRY
2011年
12期
13-13,10
,共2页
陈秀娟%苏慧英%王光琴%曾强%何念
陳秀娟%囌慧英%王光琴%曾彊%何唸
진수연%소혜영%왕광금%증강%하념
双水相萃取%荷叶%黄酮
雙水相萃取%荷葉%黃酮
쌍수상췌취%하협%황동
aqueous two-phase extraction%lotus leaves%total flavonoids
采用PEG/硫酸铵双水相体系分离纯化荷叶黄酮。实验结果表明：在PEG 4000的质量分率为25%,（NH4）2SO4的质量分率为10%,加入粗提液3 mL,在常温下,荷叶黄酮的萃取率可达97%以上。
採用PEG/硫痠銨雙水相體繫分離純化荷葉黃酮。實驗結果錶明：在PEG 4000的質量分率為25%,（NH4）2SO4的質量分率為10%,加入粗提液3 mL,在常溫下,荷葉黃酮的萃取率可達97%以上。
채용PEG/류산안쌍수상체계분리순화하협황동。실험결과표명：재PEG 4000적질량분솔위25%,（NH4）2SO4적질량분솔위10%,가입조제액3 mL,재상온하,하협황동적췌취솔가체97%이상。
A kind of aqueous two-phase system composed of PEG,（NH4）2SO4 and water was used to separate and purify total flavonoids from lotus leaves.The results showed that the best ATPS was composed of 25 % PEG4000 and 10 %（NH4）2SO4,and addition of sample was 3 mL at room temperature.Under this conditions,the yield can reach to 97 %.
