CAJ | 학술논문

建立一种肉及其制品中莱克多巴胺的酶联免疫分析方法。对莱克多巴胺残留量进行选择性（交义反应）、特异性与检测限以及回收率和精密度测试。结果显示，选择酶联免疫法来检测样品中的莱克多巴胺准确性较好，其对莱克多巴胺结构类似物的交叉反应率都小于1％，出口猪肉罐头和牛肉中的检测限（LOD）分别为（0．28±0．30）μg／kg和（0．26±0．21）μg／kg，样品甲均回收率在82．9％－91．1％之间，相对标准偏差为4．3％～8．7％，经高效液相色谱（HPLC）确证假阳性率小于等于2．0％，表明酶联免疫分析定量泫可快速、准确实现对食品中莱克多巴胺残留量的快速筛选。
건립일충육급기제품중래극다파알적매련면역분석방법。대래극다파알잔류량진행선택성（교의반응）、특이성여검측한이급회수솔화정밀도측시。결과현시，선택매련면역법래검측양품중적래극다파알준학성교호，기대래극다파알결구유사물적교차반응솔도소우1％，출구저육관두화우육중적검측한（LOD）분별위（0．28±0．30）μg／kg화（0．26±0．21）μg／kg，양품갑균회수솔재82．9％－91．1％지간，상대표준편차위4．3％～8．7％，경고효액상색보（HPLC）학증가양성솔소우등우2．0％，표명매련면역분석정량현가쾌속、준학실현대식품중래극다파알잔류량적쾌속사선。
This paper describes an enzyme-linked immunosorbent assay （ELISA） to determine ractopamine in meat and meat products. The proposed method was highly accurate in determining ractopamine and showed a cross-reactivity of less than 1% with all ractopamine analogues investigated. The limits of detection （LODs） for exported canned pork and exported beef were （0.28±0.30） μg/kg and （0.26±0.21） μg/kg, respectively, with an average spike recovery of 82.9%-91.1% （n = 15） and a relative standard deviation of 4.3%-8.7%. Using high performance liquid chromatography （HPLC）, the false posiitve rate of this method was confirmed as less than 2.0%, suggesting that it can enable accurate and rapid determination of ractopamine residues in foods.