CAJ | 학술논문

[目的]研究茉莉酸诱导的钙动员通往IP3敏感的钙离子通透性通道的作用。[方法]以低温导入法将钙离子荧光探针Fluo-3/AM导入拟南芥叶片细胞中,利用LASAF（Leica Application Suite-Advanced Fluorescence）软件记录肝素对茉莉酸（JA）诱导的胞内钙离子荧光强度的变化。[结果]经不同浓度的肝素预处理后,拟南芥叶细胞中胞内钙离子的荧光强度降低,再用100μmol/LJA处理时,其荧光强度升高,但仅与未经肝素处理的荧光强度相当。[结论]肝素预处理可抑制JA诱导的胞内钙离子浓度的升高。
[목적]연구말리산유도적개동원통왕IP3민감적개리자통투성통도적작용。[방법]이저온도입법장개리자형광탐침Fluo-3/AM도입의남개협편세포중,이용LASAF（Leica Application Suite-Advanced Fluorescence）연건기록간소대말리산（JA）유도적포내개리자형광강도적변화。[결과]경불동농도적간소예처리후,의남개협세포중포내개리자적형광강도강저,재용100μmol/LJA처리시,기형광강도승고,단부여미경간소처리적형광강도상당。[결론]간소예처리가억제JA유도적포내개리자농도적승고。
[Objective] This study was to investigate the role of IP3 sensitive calcium channel in the JA-induced calcium mobilization pathway.[Method] Arabidopsis thaliana leaves were labeled by Fluorescent probe Fluo-3/AM under low temperature at 4 ℃ to measure the fluorescent intensity of intracellular Ca2＋ which was pretreated with heparin on jasmonic acid（JA）-induced.[Results] When A.thaliana leaf cells were pretreated with 10,50 or 100 ng/ml heparin,intercellular free Ca2＋ fluorescence intensity was reduced in comparison with negative control.Once the heparin-pretreated A.thaliana leaf cells were stimulated with 100 μmol/L JA,intercellular Ca2＋ fluorescence intensity increased gradually and tended to be stable at a degree equivalent with that in negative control.[Conclusion] The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca2＋ concentration significantly which JA-induced in leaves of Arabidopsis thaliana.