农业科学与技术:英文版
農業科學與技術:英文版
농업과학여기술:영문판
Agricultural Science & Technology
2011年
6期
815-817,875
,共4页
洪玉%杨永军%常登龙%朱利泉
洪玉%楊永軍%常登龍%硃利泉
홍옥%양영군%상등룡%주리천
酵母菌%单倍体%二倍体%特性
酵母菌%單倍體%二倍體%特性
효모균%단배체%이배체%특성
Yeast%Haploid%Diploid%Characteristics
[目的]鉴别单、二倍体酵母菌株。[方法]分析了2种倍性酵母菌的特征及其联系,对酵母菌体进行美兰染色、产孢后子囊孢子的番红染色以及低温特殊处理试验,对单、二倍体酵母进行区分与鉴定。[结果]酵母菌株经美兰染色后能清晰观察到两者的活性及其菌体大小的差异;产孢后的二倍体菌株,子囊孢子被染成红色,菌体为蓝色,而单倍体菌株只有蓝色的菌体;低温特别处理后,在长有单、二倍体混合菌株的平板上,二倍体的菌落明显大于单倍体。[结论]单、二倍体酵母菌在形态上、遗传上和生理上存在很大差异,可以借助一些辅助试验很好地把它们区分开来。
[目的]鑒彆單、二倍體酵母菌株。[方法]分析瞭2種倍性酵母菌的特徵及其聯繫,對酵母菌體進行美蘭染色、產孢後子囊孢子的番紅染色以及低溫特殊處理試驗,對單、二倍體酵母進行區分與鑒定。[結果]酵母菌株經美蘭染色後能清晰觀察到兩者的活性及其菌體大小的差異;產孢後的二倍體菌株,子囊孢子被染成紅色,菌體為藍色,而單倍體菌株隻有藍色的菌體;低溫特彆處理後,在長有單、二倍體混閤菌株的平闆上,二倍體的菌落明顯大于單倍體。[結論]單、二倍體酵母菌在形態上、遺傳上和生理上存在很大差異,可以藉助一些輔助試驗很好地把它們區分開來。
[목적]감별단、이배체효모균주。[방법]분석료2충배성효모균적특정급기련계,대효모균체진행미란염색、산포후자낭포자적번홍염색이급저온특수처리시험,대단、이배체효모진행구분여감정。[결과]효모균주경미란염색후능청석관찰도량자적활성급기균체대소적차이;산포후적이배체균주,자낭포자피염성홍색,균체위람색,이단배체균주지유람색적균체;저온특별처리후,재장유단、이배체혼합균주적평판상,이배체적균락명현대우단배체。[결론]단、이배체효모균재형태상、유전상화생리상존재흔대차이,가이차조일사보조시험흔호지파타문구분개래。
[Objective] The aim was to indentify haploid and diploid yeast.[Method] The characteristics of haploid and diploid yeast and their relation were analyzed firstly,and then haploid and diploid yeast were indentified through the methylene blue staining,safranine staining of ascospore and special low temperature treatment test.[Result] After methylene blue staining,the differences of haploid and diploid yeast in activity and size could be observed clearly;the ascospores of diploid strains after sporulating became red after safranine staining,and diploid thalluses were blue,while there were only blue thalluses for haploid strains;after special low temperature treatment,the colony of diploid was obviously larger than that of haploid on the plate.[Conclusion] Haploid and diploid yeast had great differences in morphology,heredity and physiology and could be identified well by means of aided test.