农业科学与技术：英文版
農業科學與技術：英文版
농업과학여기술：영문판
Agricultural Science ＆ Technology
2011年
7期
1031-1034
,共4页
许橙%薛文志%赵茂鑫%陈信%刘颖%于汪洋%许艳丽%马腾壑%高妍%张嘉保%袁宝
許橙%薛文誌%趙茂鑫%陳信%劉穎%于汪洋%許豔麗%馬騰壑%高妍%張嘉保%袁寶
허등%설문지%조무흠%진신%류영%우왕양%허염려%마등학%고연%장가보%원보
miRNA%实时荧光定量PCR%差异表达
miRNA%實時熒光定量PCR%差異錶達
miRNA%실시형광정량PCR%차이표체
miRNA%Real-time quantitative RT-PCR%Differential expression
[目的]探索miRNA-181b在成熟（18月龄）和未成熟（1月龄）牛腺垂体中差异表达量及其调控功能。[方法]通过构建成熟（18月龄）和未成熟（1月龄）的延边黄牛腺垂体组织miRNAcDNA文库,对文库中的miRNA进行Solexa高通量深度测序,并对公牛腺垂体中的miRNA进行鉴别。从测序结果中挑选出具有差异表达的miRNA-181b,经实时荧光定量PCR验证其在不同发育期延边黄牛腺垂体中的表达规律,并通过TargetScanS预测miRNA-181b的靶基因。[结果]miRNA-181b在不同发育期牛腺垂体表达量存在显著差异,1月龄公牛腺垂体中miRNA-181b表达量是18月龄的4.05倍。miRNA181b与FSHβ基因3 非编码区838 ~844碱基可能是特异结合,其结合碱基为UGAAUGUA。[结论]该研究为FSHβ的转录后调控研究提供了理论依据。
[目的]探索miRNA-181b在成熟（18月齡）和未成熟（1月齡）牛腺垂體中差異錶達量及其調控功能。[方法]通過構建成熟（18月齡）和未成熟（1月齡）的延邊黃牛腺垂體組織miRNAcDNA文庫,對文庫中的miRNA進行Solexa高通量深度測序,併對公牛腺垂體中的miRNA進行鑒彆。從測序結果中挑選齣具有差異錶達的miRNA-181b,經實時熒光定量PCR驗證其在不同髮育期延邊黃牛腺垂體中的錶達規律,併通過TargetScanS預測miRNA-181b的靶基因。[結果]miRNA-181b在不同髮育期牛腺垂體錶達量存在顯著差異,1月齡公牛腺垂體中miRNA-181b錶達量是18月齡的4.05倍。miRNA181b與FSHβ基因3 非編碼區838 ~844堿基可能是特異結閤,其結閤堿基為UGAAUGUA。[結論]該研究為FSHβ的轉錄後調控研究提供瞭理論依據。
[목적]탐색miRNA-181b재성숙（18월령）화미성숙（1월령）우선수체중차이표체량급기조공공능。[방법]통과구건성숙（18월령）화미성숙（1월령）적연변황우선수체조직miRNAcDNA문고,대문고중적miRNA진행Solexa고통량심도측서,병대공우선수체중적miRNA진행감별。종측서결과중도선출구유차이표체적miRNA-181b,경실시형광정량PCR험증기재불동발육기연변황우선수체중적표체규률,병통과TargetScanS예측miRNA-181b적파기인。[결과]miRNA-181b재불동발육기우선수체표체량존재현저차이,1월령공우선수체중miRNA-181b표체량시18월령적4.05배。miRNA181b여FSHβ기인3 비편마구838 ~844감기가능시특이결합,기결합감기위UGAAUGUA。[결론]해연구위FSHβ적전록후조공연구제공료이론의거。
[Objective] The research aimed to discuss the differential expression quantity of miRNA-181b in mature（18-month-old） and immature（one-month-old） cattle＇s anterior pituitary and its regulation function.[Method] cDNA library of miRNA in mature（18-month-old） and immature（one-month-old） cattle＇s anterior pituitary were established.After Solexa high-throughput sequencing of miRNA in the cDNA library,miRNA in anterior pituitary of bulls was identified.miRNA-181b with differential expression were selected from the sequencing results.By real-time quantitative RT-PCR,the expression laws of miRNA-181b in the anterior pituitary of Yanbian Cattle in different growth period was validated.And the target genes of miRNA-181b were forecast by using TargetScanS prediction software.[Result] The expression quantity of miRNA-181b had great difference in cattle＇s anterior pituitary different growth periods.The expression quantity of miRNA-181b in anterior pituitary of one-month-old cattle was 4.05 times as that in 18-month-old cattle.The binding of miRNA-181b with 838-844 bases in 3＇ untranslated region of FSHβ gene was specific and the binding base sites were UGAAUGUA.[Conclusion] This research provided the theoretical basis for the transcription regulation research of FSHβ.
