CAJ | 학술논문

原子力显微镜作为第三代显微探测工具,具有原子级的空间分辨率,其样品制备方法简单易行,可在离体的近生理条件下直接观测生物样品及其动态变化过程,能够对样品进行力学操纵,在观察生物大分子的结构和生物力学特性上具有显著的优势。本文尝试从蛋白质、核酸、多糖的超微结构和力学特性的研究角度入手,期望向读者展现出原子力显微镜在大分子生物学研究中的应用前景。
원자력현미경작위제삼대현미탐측공구,구유원자급적공간분변솔,기양품제비방법간단역행,가재리체적근생리조건하직접관측생물양품급기동태변화과정,능구대양품진행역학조종,재관찰생물대분자적결구화생물역학특성상구유현저적우세。본문상시종단백질、핵산、다당적초미결구화역학특성적연구각도입수,기망향독자전현출원자력현미경재대분자생물학연구중적응용전경。
As a third-generation microscopy, atomic force microscope （AFM） has the atomic-scale spatial resolution, the simple sample preparation method and can be operated under physiological conditions to directly observe ultrastructure and dynamic process of biological samples in vitro. AFM can be used to do mechanical manipulation and observation of biomacromolecules on the structure and biomechanical properties. This paper attempts to introduce AFM applications in ultrastructure and mechanical properties of proteins, nucleic acids, polysaccharides angles to the potential readers.