CAJ | 학술논문

1988年3月～1992年2月间用不同的激光器辐照近16200个沙田柚芽，低位高接到三个小气候地区的320多株10年左右树龄的沙田柚结果树上，经栽培得到无核少核果实。对L1、L2无核果枝条的嫩叶进行过氧化物同工酶(POD)和脂酶同工酶(EST)分析、花粉育性检测，对L3代嫩叶进行RAPD研究，表明诱变组与对照组相比，存在酶活性、花粉育性、DNA分子水平上的差异，说明激光诱变引起的性状改变是可以遗传的。
1988년3월～1992년2월간용불동적격광기복조근16200개사전유아，저위고접도삼개소기후지구적320다주10년좌우수령적사전유결과수상，경재배득도무핵소핵과실。대L1、L2무핵과지조적눈협진행과양화물동공매(POD)화지매동공매(EST)분석、화분육성검측，대L3대눈협진행RAPD연구，표명유변조여대조조상비，존재매활성、화분육성、DNA분자수평상적차이，설명격광유변인기적성상개변시가이유전적。
From 1988 to 1992 about 16200 buds of Shantian Pumello had been radiated by laser.Then they had been grafted onto 320 Shantian Pumello trees.These tree's age were about 10 years old and they were distributed over three region of different longitude and latitude.The seedless or pauciseed Shatian Pumello fruit were been gained.The analysis of isoenzyme,the measuring of ratio of pollen abortion for L1 and L2 generation,the random amplified polymorphic DNA(RAPD) analysis for tender leaves of L3 generation,show that the mutant group compared with CK,have difference in isoenzyme activity,pollen abortion and RAPD.It demonstrated that the variation of Shatian Pumello induced by laser may be heritable.