CAJ | 학술논문

本研究旨在探讨锰对公鸡肝脏细胞色素P450酶系的影响.50日龄海兰褐公鸡400只,随机分为4组,分别在饲料中添加0、600、900、1 800 mg·kg~(-1)MnCl_2建立亚慢性锰中毒模型,于30、60、90 d采取肝脏检测肝微粒体细胞色素P450酶系活性以及CYP2H1基因转录水平的变化.结果显示,细胞色素P450、b_5含量,氨基吡啉-N-脱甲基酶(AND)和苯胺-4-羟化酶(AH)活性在各个时间点随染锰剂量的增加,基本呈降低趋势,且高剂量组均极显著(P<0.01)低于正常组,低、中剂量组有高于正常组的情况.NADPH-细胞色素C还原酶(CR)和红霉素-N-脱甲基酶(ERND)的活性的变化规律不明显,30和60 d NADPH-细胞色素C还原酶活性呈降低趋势,而90 d时呈升高趋势,且高剂量组极显著(P<0.01)高于正常组.红霉素-N-脱甲基酶在30 d时呈升高趋势,60和90 d时呈波动性降低变化,且高剂量组均极显著(P<0.01)低于正常组.在各个时间点,CYP2H1 mRNA的转录水平除30 d低、高剂最组,60 d中剂量组,90 d低剂量组高于正常组外,其他组均低于正常组.结果提示,锰中毒可影响鸡肝脏细胞色素P450酶系以及CYP2H1 mRNA的转录水平.
본연구지재탐토맹대공계간장세포색소P450매계적영향.50일령해란갈공계400지,수궤분위4조,분별재사료중첨가0、600、900、1 800 mg·kg~(-1)MnCl_2건립아만성맹중독모형,우30、60、90 d채취간장검측간미립체세포색소P450매계활성이급CYP2H1기인전록수평적변화.결과현시,세포색소P450、b_5함량,안기필람-N-탈갑기매(AND)화분알-4-간화매(AH)활성재각개시간점수염맹제량적증가,기본정강저추세,차고제량조균겁현저(P<0.01)저우정상조,저、중제량조유고우정상조적정황.NADPH-세포색소C환원매(CR)화홍매소-N-탈갑기매(ERND)적활성적변화규률불명현,30화60 d NADPH-세포색소C환원매활성정강저추세,이90 d시정승고추세,차고제량조겁현저(P<0.01)고우정상조.홍매소-N-탈갑기매재30 d시정승고추세,60화90 d시정파동성강저변화,차고제량조균겁현저(P<0.01)저우정상조.재각개시간점,CYP2H1 mRNA적전록수평제30 d저、고제최조,60 d중제량조,90 d저제량조고우정상조외,기타조균저우정상조.결과제시,맹중독가영향계간장세포색소P450매계이급CYP2H1 mRNA적전록수평.
The aim of this study was to investigate the effect of manganese on the hepatic cyto-chrome P450 enzyme system in cocks. 400 fifty-day old Hy-line brown cocks were randomly di-vided into four groups. The cocks in four groups were respectively fed with the basal diet contai-ning 0, 600, 900 and 1 800 mg·kg~(-1) MnCl_2 to establish the sub-chronic manganism model. After 30, 60 and 90-day treatment, the livers in every group were collected to detect the activity of microsomal cytochrome P450 enzyme system and the transcription level of CYP2H1 gene. The contents of cytochrome P450 and b_5, the activities of aminopyrin-N-demethylase(AND) and aniline4-hydroxylase(AH) were gradually declined with the increase of manganese in diet. The high dose group was significantly lower than the control group while there were no difference between the low and middle dose group and the control group. There were no markedly changes in the activities of NADPH-cytochrome C reductase(CR) and erythromycin-N-demethylase(ERND). The activity of CR declined at 30 and 60 d, but increased at 90 d. Besides, the CR activity of the high dose group was significantly higher than that in the control group(P<0.01). The activity of ERND increased at 30 d but no markedly tendency appeared at 60 and 90 d. The ERND activity in the high dose group was significantly higher than that in the low dose group(P<0.01). The transcription level of CYP2H1 mRNA in the low dose and high dose group at 30 d, the middle dose group at 60 d, and the low dose group at 90 d were higher than that in the control group.The transcription level of CYP2H1 mRNA in other treatment groups were lower than that in the control group. These results indicated that manganism could significantly changes the activities of microsomal-cytochrome P450s and the transcription level of CYP2H1 mRNA.