土壤
土壤
토양
SOILS
2009年
5期
757-763
,共7页
聚磷菌%筛选%鉴定%聚磷特性
聚燐菌%篩選%鑒定%聚燐特性
취린균%사선%감정%취린특성
Phosphate accumulating bacterial%Screening%Identification%Accumulating phosphate characteristic
采用纯培养结合蓝白斑筛选法从巢湖和南淝河底泥中分离筛选出能聚磷(P)的 11 株解 P 细菌,好氧培养时菌体吸 P 能力测定结果表明,GP44 的菌体含 P 量达到 11.92%,具有较高的聚 P 能力,对其初步鉴定为鉴定菌株 GP44 属肠杆菌科中的克雷伯氏菌属土生克雷伯氏菌(K.terrigena).GP44 在废水合成培养基上最佳聚 P 温度 30℃、初始 pH 为 7.5、最佳装液量为 120 ml/250 ml 和最适 C 源是葡萄糖,Mg~(2+)、K~+ 和 Fe~(3+) 有利于菌株 GP44 的生物除 P.
採用純培養結閤藍白斑篩選法從巢湖和南淝河底泥中分離篩選齣能聚燐(P)的 11 株解 P 細菌,好氧培養時菌體吸 P 能力測定結果錶明,GP44 的菌體含 P 量達到 11.92%,具有較高的聚 P 能力,對其初步鑒定為鑒定菌株 GP44 屬腸桿菌科中的剋雷伯氏菌屬土生剋雷伯氏菌(K.terrigena).GP44 在廢水閤成培養基上最佳聚 P 溫度 30℃、初始 pH 為 7.5、最佳裝液量為 120 ml/250 ml 和最適 C 源是葡萄糖,Mg~(2+)、K~+ 和 Fe~(3+) 有利于菌株 GP44 的生物除 P.
채용순배양결합람백반사선법종소호화남비하저니중분리사선출능취린(P)적 11 주해 P 세균,호양배양시균체흡 P 능력측정결과표명,GP44 적균체함 P 량체도 11.92%,구유교고적취 P 능력,대기초보감정위감정균주 GP44 속장간균과중적극뢰백씨균속토생극뢰백씨균(K.terrigena).GP44 재폐수합성배양기상최가취 P 온도 30℃、초시 pH 위 7.5、최가장액량위 120 ml/250 ml 화최괄 C 원시포도당,Mg~(2+)、K~+ 화 Fe~(3+) 유리우균주 GP44 적생물제 P.
Eleven kinds of bacteria with high capability of accumulating poly-P were isolated and screened from the bottom muds of Chaohu Lake and South Feihe River by using the pure culture and blue and white colored screening methods. Their phosphate accumulating capacities were investigated aerobically in synthesized sewage medium. The results showed that the total phosphorus in the GP44 cells was 11.92%, indicating a higher level of phosphorus accumulation, and it was identified as K.terrigena. GP44 grew in synthesized sewage medium with the optimal phosphate accumulating temperature, initial pH value, aeration ration and carbon source was 30℃, 7.5, 120 ml/250 ml and glucose respectively, and Mg~(2+), K~+ and Fe~(3+) could promote GP44 to accumulate phosphorus.
