水产科学
水產科學
수산과학
FISHERIES SCIENCE
2009年
12期
717-720
,共4页
花(鱼骨)%细菌性败血症%嗜水气单胞菌%药物敏感试验%抑菌试验
花(魚骨)%細菌性敗血癥%嗜水氣單胞菌%藥物敏感試驗%抑菌試驗
화(어골)%세균성패혈증%기수기단포균%약물민감시험%억균시험
Aemibarbus maculates%bacterial septicemia%Aeromonas hydrophila%medicinal sensitive test%bacteriostasis bacterial test.
利用常规分离方法对濒死病鱼的肝、肾组织进行细菌性病原分离,并对所获得的纯培养物进行VITEK-32鉴定、人工感染及体外敏感性试验.结果显示,嗜水气单胞菌是导致花鱼 骨败血症的病原菌,它对四环素、强力霉素耐药,对复方新诺明中度敏感,对恩诺沙星、氟派酸、庆大霉素、丁胺卡那霉素敏感;盐酸恩诺沙星、氟本尼考、甲砜霉素、烟酸诺氟沙星4种药物对分离菌株的最低抑菌质量浓度分别为0.065、4.0、8.0、1.0 μg/ml.
利用常規分離方法對瀕死病魚的肝、腎組織進行細菌性病原分離,併對所穫得的純培養物進行VITEK-32鑒定、人工感染及體外敏感性試驗.結果顯示,嗜水氣單胞菌是導緻花魚 骨敗血癥的病原菌,它對四環素、彊力黴素耐藥,對複方新諾明中度敏感,對恩諾沙星、氟派痠、慶大黴素、丁胺卡那黴素敏感;鹽痠恩諾沙星、氟本尼攷、甲砜黴素、煙痠諾氟沙星4種藥物對分離菌株的最低抑菌質量濃度分彆為0.065、4.0、8.0、1.0 μg/ml.
이용상규분리방법대빈사병어적간、신조직진행세균성병원분리,병대소획득적순배양물진행VITEK-32감정、인공감염급체외민감성시험.결과현시,기수기단포균시도치화어 골패혈증적병원균,타대사배소、강력매소내약,대복방신낙명중도민감,대은낙사성、불파산、경대매소、정알잡나매소민감;염산은낙사성、불본니고、갑풍매소、연산낙불사성4충약물대분리균주적최저억균질량농도분별위0.065、4.0、8.0、1.0 μg/ml.
Pathogen of the Bacterial Spticemia from Aemibarbus maculates was isolated by routinely microorganism isolation procedure, and was identified with VITEK-32 as well as artificial infection test. It indicated that Aeromonas hydrophila was the major pathogen of the Septicemia. Susceptibility Test showed that the bacterium Aeromonas hydrophila was resistant to Doxycycline,and Tetracycline, Moderate sensitive to Trimeth-sulfa and highly sensitive to Enrofloxacin,Norfloxacin,Gentamicin,and Amikacin.Another bacteriostasis test in vitro showed that Enrofloxacin,Florfenicol,Thiamphenicol and Norfloxacin had strong inhibition activity to detected pathogen,and that their MIC were 0.065,4.0,8.0,and 1.0 μg/ml,respectively.
