CAJ | 학술논문

目的探讨P-选择素(P-selectin)启动子区C-2123G基因多态性在中国广东地区健康汉族人群中的分布特点及其在不同种族间的分布差异.方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法检测240名该地区汉族人P-选择素基因C-2123G的基因型并计算其基因型频率及等位基因频率. 结果 P-选择素C-2123G基因型以GG型发生频率最高,各位点各基因型频率:CC型8.33%,CG型42.5%,GG型49.17%;C,G各等位基因频率分别为29.58%,70.42%,这种基因多态性分布在男女间无显著性差异(P>0.05).与德国、英国和泰国人群比较,发现不同种族间P-选择素C-2123G基因型分布及等位基因频率均存在显著差异(P<0.01和P<0.05);而与中国成都和湖北地区人群比较差异无显著性(P>0.05). 结论中国广东地区汉族人群中存在P-选择素启动子区C-2123G基凼多态性,在不同种族间俘在着较大的差异,这种差异可能是导致某些疾病在不同种族间的发病率和临床表现存在显著不同的因素之一.
목적 탐토P-선택소(P-selectin)계동자구C-2123G기인다태성재중국엄동지구건강한족인군중적분포특점급기재불동충족간적분포차이.방법 응용취합매련반응-한제성편단장도다태성(PCR-RFLP)분석방법검측240명해지구한족인P-선택소기인C-2123G적기인형병계산기기인형빈솔급등위기인빈솔. 결과 P-선택소C-2123G기인형이GG형발생빈솔최고,각위점각기인형빈솔:CC형8.33%,CG형42.5%,GG형49.17%;C,G각등위기인빈솔분별위29.58%,70.42%,저충기인다태성분포재남녀간무현저성차이(P>0.05).여덕국、영국화태국인군비교,발현불동충족간P-선택소C-2123G기인형분포급등위기인빈솔균존재현저차이(P<0.01화P<0.05);이여중국성도화호북지구인군비교차이무현저성(P>0.05). 결론중국엄동지구한족인군중존재P-선택소계동자구C-2123G기당다태성,재불동충족간부재착교대적차이,저충차이가능시도치모사질병재불동충족간적발병솔화림상표현존재현저불동적인소지일.
Objective To investigate the distribution of the gene polymorphism at position C-2123G in the healthy Hart population in Guangdong China, as well as to compare distributions of genotypo and allele frequencies among different races and countries. Methods The genotype and allele frequencies of P-selectin gene in promoter region C-2123G were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) in 240 healthy Hart nationality people in Guangdong China. Results The genotype frequency of P-selectin C-2123G from healthy Hart nationality in Guangdong China were 8.33% in CC, 42.50% in CG, 49.17% in GG, respectively. The allele gene frequencies of P-selectin C-2123G were 29.58% in C, 70.42% in G, respectively. No difference was detected between male and female or between Guangdong and Chengdu or Hubei China(P>0.05). The different were detedcted in genotype of P-seleetin among ethnics and areas of German, England and Thailand(P<0.01, P<0.05). Conclusion The gene polymorphisms at position C-2123G in promoter region of P-selectin in Han population of Guangdong China were different from ethnics and areas of German, England and Thailand.