CAJ | 학술논문

目的了解多重耐药铜绿假单胞菌中β-内酰胺酶基因及膜孔蛋白基因存在状况,为临床合理使用抗菌药物提供理论依据.方法收集本院临床分离的铜绿假单胞菌共30株,采用纸片扩散法进行药敏试验;用聚合酶链反应(PCR)及序列分析方法,分析20种β-内酰胺酶基因(TEM、SHV、OXA-1群、OXA-2群、PXA-10群、PER、GES、VEB、CARB、IMP、VIM、SPM、GIM、SIM、DHA)和膜孔蛋白基因(OprD2).结果 30株多重耐药铜绿假单胞菌中TEM基因阳性28株,阳性率93.3%.CARB基因阳性1株,阳性率3.3%;膜孔蛋白编码基因OprD2缺失29株,阳件率96.7%.末检m其他β-内酰胺酶基因.结论烟台地区铜绿假单胞菌β-内酰胺酶基因(TEM)和膜孔蛋白编码基因OprDz缺失株检出率均在90%以上.
목적 료해다중내약동록가단포균중β-내선알매기인급막공단백기인존재상황,위림상합리사용항균약물제공이론의거.방법 수집본원림상분리적동록가단포균공30주,채용지편확산법진행약민시험;용취합매련반응(PCR)급서렬분석방법,분석20충β-내선알매기인(TEM、SHV、OXA-1군、OXA-2군、PXA-10군、PER、GES、VEB、CARB、IMP、VIM、SPM、GIM、SIM、DHA)화막공단백기인(OprD2).결과 30주다중내약동록가단포균중TEM기인양성28주,양성솔93.3%.CARB기인양성1주,양성솔3.3%;막공단백편마기인OprD2결실29주,양건솔96.7%.말검m기타β-내선알매기인.결론 연태지구동록가단포균β-내선알매기인(TEM)화막공단백편마기인OprDz결실주검출솔균재90%이상.
Objective To investigate the status of beta-lactamase genes and outer membrane ptoteirl gene in 30 strains of Pseudomonas aeruginosa(PA)isolated from elinical samples,so as toprovide theoretical evidence for rational use of antibiotics in clinic.Methods Totally 30 strains of PA isolated from clinical samples were collected.Slip diffusing method was applied tO performing drug susceptibility test,and polymerase chain reaction(PCR)assay and sequence analysis were adopted to investigate 20 kinds of beta-lactamase genes(TEM,SHV,0XA-1 group,OXA-10 group,PER,GES,VEB,CARB,IMP,VIM,SPM,GIM,SIM and DHA)and outer membrane protein genas (OprD2).Results The positive rate of TEM,CARB and deletion of OprD2 gene was 93.3%(28/30),3.3%(1/30)and 96.7%(29/30)respectively.No other beta-lactamase gene was detected.Conclusion There is a higher positive rate(over 90%)of.beta-lactamase genes and outer membrane protein gene deficiency in Pseudomonas aeruginosa in Yantaioistrict.