植物学报
植物學報
식물학보
ACTA BOTANICA SINICA
2001年
2期
164-168
,共5页
英加%李滨%刘建中%李振声%穆素梅%周汉平
英加%李濱%劉建中%李振聲%穆素梅%週漢平
영가%리빈%류건중%리진성%목소매%주한평
蓝粒小麦%易位系%易位附加%易位-易位附加%荧光原位杂交
藍粒小麥%易位繫%易位附加%易位-易位附加%熒光原位雜交
람립소맥%역위계%역위부가%역위-역위부가%형광원위잡교
普通小麦(Triticum aestivum L.)和长穗偃麦草(Agropyronelongatum(Host)Beauv=Elytriga elongatum(Host)Nevski=Thinopyrum ponticum(Host)Barkworth and Dewey,2n=10x=70)杂交后选育出的蓝粒小麦异代换系(蓝58),经辐射诱导与选育,对其后代进行荧光原位杂交检测后获得:(1)9902,9906两个蓝粒易位系,体细胞染色体数均为2n=42,其中9906中被易位蓝粒片段的相对长度约占易位小麦染色体短臂的1/3,而9902中被易位蓝粒片段的相对长度约占易位小麦染色体长臂的1/2,并将9902的蓝粒易位片段定位在小麦D组染色体上;(2)9915易位附加和9904易位-易位附加,其体细胞染色体数均为44,其中9915的体细胞染色体只有一对发生了易位,另外附加了两条长穗偃麦草染色体;而9904有两对染色体发生了易位。并就易位系中控制蓝粒性状的长穗偃麦草染色体片段的定位和蓝粒小麦易位系的应用进行了讨论。
普通小麥(Triticum aestivum L.)和長穗偃麥草(Agropyronelongatum(Host)Beauv=Elytriga elongatum(Host)Nevski=Thinopyrum ponticum(Host)Barkworth and Dewey,2n=10x=70)雜交後選育齣的藍粒小麥異代換繫(藍58),經輻射誘導與選育,對其後代進行熒光原位雜交檢測後穫得:(1)9902,9906兩箇藍粒易位繫,體細胞染色體數均為2n=42,其中9906中被易位藍粒片段的相對長度約佔易位小麥染色體短臂的1/3,而9902中被易位藍粒片段的相對長度約佔易位小麥染色體長臂的1/2,併將9902的藍粒易位片段定位在小麥D組染色體上;(2)9915易位附加和9904易位-易位附加,其體細胞染色體數均為44,其中9915的體細胞染色體隻有一對髮生瞭易位,另外附加瞭兩條長穗偃麥草染色體;而9904有兩對染色體髮生瞭易位。併就易位繫中控製藍粒性狀的長穗偃麥草染色體片段的定位和藍粒小麥易位繫的應用進行瞭討論。
보통소맥(Triticum aestivum L.)화장수언맥초(Agropyronelongatum(Host)Beauv=Elytriga elongatum(Host)Nevski=Thinopyrum ponticum(Host)Barkworth and Dewey,2n=10x=70)잡교후선육출적람립소맥이대환계(람58),경복사유도여선육,대기후대진행형광원위잡교검측후획득:(1)9902,9906량개람립역위계,체세포염색체수균위2n=42,기중9906중피역위람립편단적상대장도약점역위소맥염색체단비적1/3,이9902중피역위람립편단적상대장도약점역위소맥염색체장비적1/2,병장9902적람립역위편단정위재소맥D조염색체상;(2)9915역위부가화9904역위-역위부가,기체세포염색체수균위44,기중9915적체세포염색체지유일대발생료역위,령외부가료량조장수언맥초염색체;이9904유량대염색체발생료역위。병취역위계중공제람립성상적장수언맥초염색체편단적정위화람립소맥역위계적응용진행료토론。
The blue-grained wheat substitution line (blue 58) originatedfrom wild hybridization between Triticum aestivum L. and Agropyron elongatum (Host) Beauv=Elytrigia elongatum (Host) Nevski=Thinopyrum ponticum (Host) Barkworth and Dewey (2n=10x=70) was irradiated and four translocation lines were screened by fluorescence in situ hybridization from the offsprings. The results obtained include the following: (1) both the two translocation lines, 9906 and 9902, have 42 chromosomes. The length of the translocated blue-grained segment was approximately one-third of the short-arm and one-half of the long-arm of the translocated wheat chromosome in 9906 and 9902, respectively, and the blue-grained translocated segment in 9902 was located on D genome; (2) both 9915 and 9904 have 44 chromosomes. One pair of chromosomes was translocated and two chromosomes from Th. ponticum were added in 9903, while two pairs of chromosomes were translocated in 9904 by blue-grained wheat segment. The location and application of blue-grained wheat translocation lines were discussed.