遗传学报
遺傳學報
유전학보
ACTA GENETICA SINICA
2001年
2期
158-165
,共8页
蔡从利%王建波%景润春%朱英国
蔡從利%王建波%景潤春%硃英國
채종리%왕건파%경윤춘%주영국
山羊草属%异源多倍体%基因组进化%RAPD
山羊草屬%異源多倍體%基因組進化%RAPD
산양초속%이원다배체%기인조진화%RAPD
用24个随机引物对山羊草属(Aegilops L.)异源多倍体物种及其祖先二倍体物种进行RAPD分析。对扩增出的313条带进行聚类分析发现,含D基因组的多倍体与二倍体祖先Ae.squarrosa(DD)在聚类图上聚为一支;除Ae. juvenalis (DDMMUU)聚到上一支外,含U基因组的多倍体与二倍体祖先Ae. umbellulata(UU)在聚类图上聚为另一支;多倍体与其他二倍体均不聚在一起,表明多倍体分别与Ae. squarrosa(DD)、Ae. umbellulata(UU)具有较近的亲缘关系。这说明多倍体形成之后,D和U基因组变化较小,而其他基因组则发生了较大的变化。
用24箇隨機引物對山羊草屬(Aegilops L.)異源多倍體物種及其祖先二倍體物種進行RAPD分析。對擴增齣的313條帶進行聚類分析髮現,含D基因組的多倍體與二倍體祖先Ae.squarrosa(DD)在聚類圖上聚為一支;除Ae. juvenalis (DDMMUU)聚到上一支外,含U基因組的多倍體與二倍體祖先Ae. umbellulata(UU)在聚類圖上聚為另一支;多倍體與其他二倍體均不聚在一起,錶明多倍體分彆與Ae. squarrosa(DD)、Ae. umbellulata(UU)具有較近的親緣關繫。這說明多倍體形成之後,D和U基因組變化較小,而其他基因組則髮生瞭較大的變化。
용24개수궤인물대산양초속(Aegilops L.)이원다배체물충급기조선이배체물충진행RAPD분석。대확증출적313조대진행취류분석발현,함D기인조적다배체여이배체조선Ae.squarrosa(DD)재취류도상취위일지;제Ae. juvenalis (DDMMUU)취도상일지외,함U기인조적다배체여이배체조선Ae. umbellulata(UU)재취류도상취위령일지;다배체여기타이배체균불취재일기,표명다배체분별여Ae. squarrosa(DD)、Ae. umbellulata(UU)구유교근적친연관계。저설명다배체형성지후,D화U기인조변화교소,이기타기인조칙발생료교대적변화。
RAPD analysis was performed by applying a set of 24 arbitrary rimers to all the allopolyploids and their diploid progenitors in Aegilops L. The analysis based on 313 RAPD fragments revealed that the allopolyploids carrying D genome grouped with Ae. squarrosa (DD) in one cluster. The allopolyploids carrying U genome grouped with Ae.umbellulata (UU) in another cluster except Ae. juvenalis (DDMMUU),which grouped with Ae. squarrosa (DD). The allopolyploids did not cluster with the other diploids. The results indicated that the allopolyploids shared close relationship with Ae. squarrosa (DD) and Ae. umbellulata (UU), respectively. It proved that the D and U genomes altered relatively little from the diploid progenitors after the allopolyploids came into being, but the other genomes changed greatly.