CAJ | 학술논문

通过对5种分离根结线虫寄生真菌的方法的比较，发现通过雌成虫平板分离法和卵平板分离法有较高的成功率，其他三种自幼虫上或土壤中分离的方法很少取得成功。从华东地区104个根结线虫样品中分离得到根结线虫寄生真菌638个菌株，鉴定为14个属22个种，其中Cylindrocarpondestructans,C.heteronema,Fusariumequiseti,F.lateritium,F.proliferatum,Gliocladiumvirens,Humicolafuscoatra,Idriellalunata,Trichodermahamatum,T.harzianum,Verticilliumcatenulatum,Volutellaciliata为首次报道在根结线虫上寄生。通过种群分析发现Fusariumsolani出现频率最高，F.oxysporum，Paceilomyceslilacinus和Acremoniumstrictum为常见种，这些种在根结线虫上广泛分布。同时还发现卵和成虫寄生真菌有所不同，Gliocladiumvirens和Trichodermaharzianum仅在卵上分离到，而Volutellaciliata,Trichodermahamatum和Idriellalunata仅在雌成虫分离到，从幼虫上仅分离一次Paecilomyceslilacinus。其它真菌在卵和成虫上均可分离得到，真菌种类在三种根结线虫上和在不同地区有所不同
통과대5충분리근결선충기생진균적방법적비교，발현통과자성충평판분리법화란평판분리법유교고적성공솔，기타삼충자유충상혹토양중분리적방법흔소취득성공。종화동지구104개근결선충양품중분리득도근결선충기생진균638개균주，감정위14개속22개충，기중Cylindrocarpondestructans,C.heteronema,Fusariumequiseti,F.lateritium,F.proliferatum,Gliocladiumvirens,Humicolafuscoatra,Idriellalunata,Trichodermahamatum,T.harzianum,Verticilliumcatenulatum,Volutellaciliata위수차보도재근결선충상기생。통과충군분석발현Fusariumsolani출현빈솔최고，F.oxysporum，Paceilomyceslilacinus화Acremoniumstrictum위상견충，저사충재근결선충상엄범분포。동시환발현란화성충기생진균유소불동，Gliocladiumvirens화Trichodermaharzianum부재란상분리도，이Volutellaciliata,Trichodermahamatum화Idriellalunata부재자성충분리도，종유충상부분리일차Paecilomyceslilacinus。기타진균재란화성충상균가분리득도，진균충류재삼충근결선충상화재불동지구유소불동
The comparison of five isolation methods （Baermann funnel method,differential centrifugation method, Drechsler/s method ,female plate method and egg plate method）of the parasitic fungi of root-knot nematodes indicated that the female plate method and egg plate method were much successive than three other methods from juveniles and soils. 638 isolates of parasitic fungi from 104 samples of root-knot nematodes in six provinces of East China were identified as 22 species of 14 genera, in which 12 species were first found to be parasites of the root-knot nematodes. Fusarium solani was the prominent species , F. oxysporum, Paecilomyces lilacinus, and Acremonium strictum were frequent species. There were different fungal species on eggs and on females. Gliocladium rivens and Trichoderma harzianum were only found on eggs; Volutella ciliata, T.hamatum and Idriella lunata only on females; Paecilomyces lilacinus only once on juveniles.