CAJ | 학술논문

目的检测猪囊尾蚴胶原蛋白的抗原性。方法经不同盐浓度抽提出Ⅰ、Ⅲ、Ⅴ三型胶原蛋白，其中Ⅲ、Ⅴ型是首次从猪囊尾蚴抽提出来的，用间接ELISA法检测其抗原性。结果Ⅰ、Ⅲ、Ⅴ型胶原蛋白测35例脑囊虫病患者IgG抗体的阳性率分别为88.6%(31/35)、82.8%(29/35)、97.1%(34/35)，30例正常人均为阴性。结论猪囊尾蚴胶原蛋白具有很强的抗原性，是一组有望成为囊虫病免疫诊断用的新抗原蛋白。
목적검측저낭미유효원단백적항원성。방법경불동염농도추제출Ⅰ、Ⅲ、Ⅴ삼형효원단백，기중Ⅲ、Ⅴ형시수차종저낭미유추제출래적，용간접ELISA법검측기항원성。결과Ⅰ、Ⅲ、Ⅴ형효원단백측35례뇌낭충병환자IgG항체적양성솔분별위88.6%(31/35)、82.8%(29/35)、97.1%(34/35)，30례정상인균위음성。결론저낭미유효원단백구유흔강적항원성，시일조유망성위낭충병면역진단용적신항원단백。
Objective　To detect antigenicity of collagen of cysticarcus cellulosae.Methods　Three type collagens(Ⅰ，Ⅲ,Ⅴ)were extracted through different concentration of sodium solusion.The Ⅲ,Ⅴ type collagens were extracted from cysticercus for first time.The antigenicity of them was tested by indirect-ELISA.Results　The positive rate of IgG in the serum of 35 cysticercosis patients was taken on different results,measured by the three type collagens,which were Ⅰ 88.6%(31/35),Ⅲ 82.8%(29/35),Ⅴ 97.1%(34/35) respectively.In contrast,the control group was negative in all.Conclusion　The collagens of cysticercus cellulosae possess the intensive antigenicity,it will be a group of prospective antigens in cysticercosis immunodiagnosis.