CAJ | 학술논문

实验用鸟经脑灌流后于RA 取材,用Epon 812包埋剂浸润包埋,经铀-铅电子染色后,H-600投射电镜下观察,IBAS图像分析仪进行处理.结果发现,燕雀RA的轴-体突触较多见.突触类型包括Gray Ⅰ型和Gray Ⅱ型.Ⅰ型突触后膜显著增厚,突触前含有清亮圆形小泡；Ⅱ型突触前后膜对称,突触小泡多为扁平囊泡.经统计学t检验,突触总数、Gray Ⅰ型突触数和棘突触数,雄鸟多于雌鸟,性别差异极显著(P<0.01).
실험용조경뇌관류후우RA 취재,용Epon 812포매제침윤포매,경유-연전자염색후,H-600투사전경하관찰,IBAS도상분석의진행처리.결과발현,연작RA적축-체돌촉교다견.돌촉류형포괄Gray Ⅰ형화Gray Ⅱ형.Ⅰ형돌촉후막현저증후,돌촉전함유청량원형소포；Ⅱ형돌촉전후막대칭,돌촉소포다위편평낭포.경통계학t검험,돌촉총수、Gray Ⅰ형돌촉수화극돌촉수,웅조다우자조,성별차이겁현저(P<0.01).
The bird brains were perfuse and RA (the robust nucleus of the archistriatum) samples were cut and embedded in Epon 812.The ultrathinsections were mounted on 200-mesh grids, double-stained with ethanolic uranyl acetate, followed by concentrated lead citrate, and examined in H-600 electron microscope. The photographs were treated on IBAS system. The results showed that axo-somatic synapse in RA is dominant. Most of synapses are Gray I, which have wider cleft and asymmetrical type with round and limpid vesicles. Fewer of synapses are Gray II, which have narrow cleft and symmetrical type with flatted vesicles. The size of RA cells is larger in male than that of in female. The statistical test showed that there is significant difference between sexes (P<0.01).