CAJ | 학술논문

用人工选育的异源四倍体鲫鲤(♂)与白鲫(♀)杂交获得具有明显生长优势的三倍体湘云鲫。采用差速离心和核酸酶处理等方法，从三倍体、白鲫和异源四倍体鲫鲤肝组织中提取线粒体DNA，并用9种限制性内切酶进行单酶酶切分析。经琼脂糖凝胶电泳后计算出各酶切片段的大小，测得三倍体、白鲫和异源四倍体鲫鲤mtDNA的分子大小分别为16.24kb、16.60kb和16.20kb。根据各单倍型间的酶切片段共享度，估算出3个群体间的遗传距离，说明了mtDNA母系遗传的特性。
용인공선육적이원사배체즉리(♂)여백즉(♀)잡교획득구유명현생장우세적삼배체상운즉。채용차속리심화핵산매처리등방법，종삼배체、백즉화이원사배체즉리간조직중제취선립체DNA，병용9충한제성내절매진행단매매절분석。경경지당응효전영후계산출각매절편단적대소，측득삼배체、백즉화이원사배체즉리mtDNA적분자대소분별위16.24kb、16.60kb화16.20kb。근거각단배형간적매절편단공향도，고산출3개군체간적유전거리，설명료mtDNA모계유전적특성。
The triploid crucian carp, with obvious advantage in growth, wasobtained by crossing allotetraploid hybrids(♂) from offsprings(F3～F9)of red crucian carp (Carassius auratus red var.)(♂) ×common carp (Cyprinus carpio L.) (♀), and C.auratus cuvieri (♀). The mitochonodrial DNA (mtDNA) of liver were isolated from the triploids, Japanese crucian carp (C. auratus cuvieri) and allotetraploid hybrids by means of density gradient centrifugation, DNase I and RNase digestion, and were digested by 9 kinds of restriction endonucleases. By analysis of agrose gel electrophoresis, the sizes of mtDNA fragments were estimated that the mtDNA sizes of triploids, C. auratuscuvieri and allotetraploid hybrids were 16.24×103, 16.60×103 and 16.30×103bp, respectively, and the genetic distances among mtDNA haploidy types in the 3 populations were calculated according to the numbers of common fragments that they had the matrilinear inheritence character of mtDNA.