CAJ | 학술논문

以籼稻品种成熟种子为材料，研究了胚乳对成熟胚愈伤组织诱导和分化的影响。结果表明，种子胚乳量愈少，始愈期愈早；形成的愈伤组织生长速率以带全胚乳的种子为最快；愈伤组织分化率则以带半胚乳的种子最高。愈伤组织继代培养次数显著影响绿苗分化频率，以继代培养1次(约12～14 d)的愈伤组织分化率最高。
이선도품충성숙충자위재료，연구료배유대성숙배유상조직유도화분화적영향。결과표명，충자배유량유소，시유기유조；형성적유상조직생장속솔이대전배유적충자위최쾌；유상조직분화솔칙이대반배유적충자최고。유상조직계대배양차수현저영향록묘분화빈솔，이계대배양1차(약12～14 d)적유상조직분화솔최고。
During the process of the culture in vitro of mature rice seed, the amount of endoeperm affected the callus formation, callus growth speed and its differentiation. The less the amount of endosperm left in seeds was, the earlier the callus formation from seed was. On the contrary, the more the amount of endosperm left in seeds, the faster the callus grew. However,the callus from seeds containing a half of endosperm was observed to regenerate more plants. Meanwhile, the number of times in the callus culture influenced frequency of the plant regeneration, the first time (abort 12 to 14 day) of the callus culture was more effective in inducing plant regenreation.