CAJ | 학술논문

以4个普通小麦同核异质系——可育系D2-CA8057（BC14）、D2型不育系msD2-CA8057（BC11）、YA型不育系msA-CA8057（BC12）、CA8057（核亲本）为材料，采用PAGE方法比较了4个系灌浆期种子胚乳和“花粉双核期”花药的过氧化物酶同工酶，采用梯度SDS-PAGE方法比较了4个系不同发育时期（干种子胚乳、越冬前和越冬后苗期叶片、“花粉双核期”旗叶、“花粉双核期”花药）可溶性蛋白质的组份，发现可育系之间差异较小，不育系与可育系之间、D2型不育系与YA型不育系之间存在显著差异。表明细胞质（不育）基因的表达具有时空性和特异性，这些差异（表达）带作为细胞质（不育）基因对核基因的特异调控表达产物，可以作为4个系的生化标记，同时也表明msD2-CA8057和msA-CA8057是2个不同的新不育类型。
이4개보통소맥동핵이질계——가육계D2-CA8057（BC14）、D2형불육계msD2-CA8057（BC11）、YA형불육계msA-CA8057（BC12）、CA8057（핵친본）위재료，채용PAGE방법비교료4개계관장기충자배유화“화분쌍핵기”화약적과양화물매동공매，채용제도SDS-PAGE방법비교료4개계불동발육시기（간충자배유、월동전화월동후묘기협편、“화분쌍핵기”기협、“화분쌍핵기”화약）가용성단백질적조빈，발현가육계지간차이교소，불육계여가육계지간、D2형불육계여YA형불육계지간존재현저차이。표명세포질（불육）기인적표체구유시공성화특이성，저사차이（표체）대작위세포질（불육）기인대핵기인적특이조공표체산물，가이작위4개계적생화표기，동시야표명msD2-CA8057화msA-CA8057시2개불동적신불육류형。
The peroxidase isozyme and the soluble peoteins of fourisonucleus-alloplasmic lines of common wheat——D2-CA8057 (fertile, BC14), CMS line msD2-CA8057(BC11), CMS line msA-CA8057(BC12), CA8057 (nucleus donor) in different developmental stages were studies by polyacrylamide gel electrophoresis (PAGE) and gradient SDS-PAGE respectively. The peroxidase isozyme of bionucleus-stage anther, milk-stage endosperm and the soluble proteins of bionucleus-stage anther, leaf (the beginning and ending overwinter), dry杝eed endosperm among fertile line, msD2-CA8057 and msA-CA8057 were evidently different (but few differences between CA8057 and D2-CA8057). These differences resulted from the pecially-regulated expression of (male-sterile) cytoplasmic genes to nuclear genes and could be used to identify the four isonucleus-alloplasmic lines. These results showed that msD2-CA8057 and msA-CA8057 were two different kinds of new CMS pattern.