CAJ | 학술논문

将带有NPT-Ⅱ基因的质粒PGV用花粉管通道途径转化小麦烟农103，共结实1025粒，其中有718粒种子可以萌发出幼苗，在这718株幼苗中，经卡那霉素筛选，得到75棵绿色抗性植株，提取这些抗性植株的叶片总DNA，作NPT-Ⅱ基因的PCR扩增，有37株为阳性，转化率为5.16％，对其中9个PCR阳性植株的Southern杂交检测结果表明它们均为转基因植株.
장대유NPT-Ⅱ기인적질립PGV용화분관통도도경전화소맥연농103，공결실1025립，기중유718립충자가이맹발출유묘，재저718주유묘중，경잡나매소사선，득도75과록색항성식주，제취저사항성식주적협편총DNA，작NPT-Ⅱ기인적PCR확증，유37주위양성，전화솔위5.16％，대기중9개PCR양성식주적Southern잡교검측결과표명타문균위전기인식주.
The plasmid PGV with NPT-Ⅱ gene was transferred into wheat C. V.Yannnong 103 by pollen tube pathway. 1025 transformed seeds were selected with kanamycin,in which 718 germinated and 75 expressed resistence to kanamycin. Resistant green plants were determined by PCR amplificaton of transgenic fragment and confirmed by southern hybridization with a transformation efficiency of 5.16％.