CAJ | 학술논문

目的　研究地塞米松、苯巴比妥钠和利多卡因预处理的昆明种小鼠P450 3A酶有无诱导作用及其作用机制.方法　给昆明种小鼠腹腔注射常用量地塞米松、苯巴比妥钠或利多卡因1 wk后，观察MEGX累积排泄量、P450 3A酶蛋白含量及其mRNA表达的改变.结果　与正常对照组相比，地塞米松组小鼠MEGX累积排泄量增加35.0%(P<0.05)，P450 3A酶蛋白含量增加147.1%(P<0.01)；苯巴比妥钠小鼠MEGX累积排泄量增加23.4%(P<0.05)，酶蛋白含量增加43.6%(P<0.01)；各组间P450 3A酶mRNA表达无明显差别.结论　地塞米松和苯巴比妥钠对昆明种小鼠肝P450 3A酶有诱导作用，该作用位于转录后水平；利多卡因对昆明种小鼠肝P450 3A酶无影响.
목적　연구지새미송、분파비타납화리다잡인예처리적곤명충소서P450 3A매유무유도작용급기작용궤제.방법　급곤명충소서복강주사상용량지새미송、분파비타납혹리다잡인1 wk후，관찰MEGX루적배설량、P450 3A매단백함량급기mRNA표체적개변.결과　여정상대조조상비，지새미송조소서MEGX루적배설량증가35.0%(P<0.05)，P450 3A매단백함량증가147.1%(P<0.01)；분파비타납소서MEGX루적배설량증가23.4%(P<0.05)，매단백함량증가43.6%(P<0.01)；각조간P450 3A매mRNA표체무명현차별.결론　지새미송화분파비타납대곤명충소서간P450 3A매유유도작용，해작용위우전록후수평；리다잡인대곤명충소서간P450 3A매무영향.
AIM　To study the induction of dexamethasone, on hepatic P450 3A enzyme in Kunming mice.METHODS　After administering ip a non-toxic dose of dexamethasone, phenobarbital or lidocaine to Kunming mice for 7 d, the urinary recovery of MEGX, a special lidocaine metabolite catalyzed by hepatic P450 3A enzyme, the content of hepatic P450 3A enzyme and its mRNA expression were analyzed by TDX, ELISA and RT-PCR techniques.RESULTS　In comparison with mice as control, the urinary recovery of MEGX increased by 35.0% and 23.4% (P<0.05); and the content of hepatic P450 3A enzyme increased by 147.1% and 43.6% (P<0.01) in mice pretreated with dexamethasone or phenobarbital, respectively, but not mRNA nor the pretreatment with lidocaine.CONCLUSION　The pretreatment with dexamethasone and phenobarbital may induce hepatic P450 3A enzyme, which occurs at post-transcription level, but lidocaine did not.