中国科学C辑(英文版)
中國科學C輯(英文版)
중국과학C집(영문판)
SCIENCE IN CHINA (Series C)
2002年
6期
569-576
,共8页
廖贡献%俞冠翘%沈善炯%朱家璧
廖貢獻%俞冠翹%瀋善炯%硃傢璧
료공헌%유관교%침선형%주가벽
bacterial two-hybrid system%NifL-NifA complex%environmental conditions%Enterobacter cloacae%Western blotting
Expression of the nitrogen fixation (nif ) genes is tightly regulated by two proteins NifA and NifL in the (-subdivision of the proteobacteria. NifA is a transcriptional activator, which can be inactivated by NifL in the presence of oxygen or excess fixed nitrogen. A direct interaction between E. cloacae NifL and NifA was detected using the bacterial two-hybrid system. This interaction was accelerated in the presence of fixed nitrogen, while oxygen had no effect. NifL proteins, with their C-terminus being deleted, completely lost the ability to interact with NifA. The data suggest that the C-terminal domain of NifL acts as a sensor of the nitrogen status of the cell and mediates interaction with NifA.
