CAJ | 학술논문

目的：研究鼻咽癌染色体 16q22 24的遗传稳定性。方法：用染色体 16q22 24上的 8对微卫星多态性标记分析 50例鼻咽癌的杂合性缺失（ loss of heterozygosity, LOH）与微卫星不稳定性（ microsatellite instability,MSI）。结果：至少一个位点发生 LOH的肿瘤占 48％ (24/50)， MSI的发生率为 18％（ 9/50）。但这些变化均散在分布，未见高频共同缺失区和微卫星不稳定区；其变化在早期（Ⅰ /Ⅱ期）和晚期（Ⅲ /Ⅳ期）病人之间有显著性差异（ P< 0.05）。结论：染色体 16q22 24区的遗传不稳定性的变化可能与鼻咽癌的发病有关，该区是否存在鼻咽癌相关基因有待进一步探讨。
목적：연구비인암염색체 16q22 24적유전은정성。방법：용염색체 16q22 24상적 8대미위성다태성표기분석 50례비인암적잡합성결실（ loss of heterozygosity, LOH）여미위성불은정성（ microsatellite instability,MSI）。결과：지소일개위점발생 LOH적종류점 48％ (24/50)， MSI적발생솔위 18％（ 9/50）。단저사변화균산재분포，미견고빈공동결실구화미위성불은정구；기변화재조기（Ⅰ /Ⅱ기）화만기（Ⅲ /Ⅳ기）병인지간유현저성차이（ P< 0.05）。결론：염색체 16q22 24구적유전불은정성적변화가능여비인암적발병유관，해구시부존재비인암상관기인유대진일보탐토。
Objective：This study was designed to evaluate the genetic stability on chromosome 16q22 24 in primary nasopharyngeal carcinoma. Methods: Tissue samples from fifty patients with nasopharyngeal carcinoma (NPC) tumors were examined by loss of heterozygosity (LOH) and microsatellite instability (MSI) analysis with a panel of 8 microsatellite polymorphic markers distributed along the chromosome 16q22 24. Results: LOH was observed at one or more loci in 24 cases (48％ ) and the prevalence of MSI was detected in 9 cases (18％ ). But the genomic alterations scattered along the region, neither common deletion or instability region was found. Conclusions: The status of genetic stability in chromosome 16q22 24 suggests that the genomic alterations on chromosome 16q22 24 may be involved in the development of NPC.