CAJ | 학술논문

应用光学显微镜和扫描电子显微镜观察了豆科Leguminosae土圞儿属Apios和旋花豆属Cochlianthus植物的叶表皮特征.在光学显微镜下,大多数种类的叶表皮细胞形状(表面观)为不规则型,垂周壁式样为浅波状或波状,只有一个种--Apios gracillima Dunn叶表皮细胞为多边形,垂周壁式样为较平直.在扫描电子显微镜下,多数种类的叶片蜡质纹饰通常为片状或颗粒状,一些种叶表皮未见蜡质纹饰;气孔器外拱盖内缘通常为浅波状或近平滑.在这两个属内,上述叶表皮特征在物种内较稳定,因此对于解决属内种问和属间关系有一定的系统学意义.本研究得出以下结论:(1)A.gracillima叶的上表皮细胞为多边形,垂周壁平直或弧形,A.delavayi Franch.叶的上表皮细胞为不规则形,垂周壁波状,二者存在明显差异,因此A.gracillima应处理为一独立的种,而不是A.delavayi下的一个变种;(2)A.priceana B.L.Rob.在叶表皮细胞形状、垂周壁式样、气孔器类型和蜡质纹饰形态等方面与土圞儿属中其他种不存在显著区别,因此土圞儿属内两个亚属的划分是不合理的;(3)土圞儿属多为5或7小叶,无毛或近无毛,叶表皮细胞垂周壁波状或近平直,而旋花豆属3小叶,密被柔毛,叶表皮细胞垂周壁浅波状,本文支持它们为两个独立的属的分类处理.
응용광학현미경화소묘전자현미경관찰료두과Leguminosae토란인속Apios화선화두속Cochlianthus식물적협표피특정.재광학현미경하,대다수충류적협표피세포형상(표면관)위불규칙형,수주벽식양위천파상혹파상,지유일개충--Apios gracillima Dunn협표피세포위다변형,수주벽식양위교평직.재소묘전자현미경하,다수충류적협편사질문식통상위편상혹과립상,일사충협표피미견사질문식;기공기외공개내연통상위천파상혹근평활.재저량개속내,상술협표피특정재물충내교은정,인차대우해결속내충문화속간관계유일정적계통학의의.본연구득출이하결론:(1)A.gracillima협적상표피세포위다변형,수주벽평직혹호형,A.delavayi Franch.협적상표피세포위불규칙형,수주벽파상,이자존재명현차이,인차A.gracillima응처리위일독립적충,이불시A.delavayi하적일개변충;(2)A.priceana B.L.Rob.재협표피세포형상、수주벽식양、기공기류형화사질문식형태등방면여토란인속중기타충불존재현저구별,인차토란인속내량개아속적화분시불합리적;(3)토란인속다위5혹7소협,무모혹근무모,협표피세포수주벽파상혹근평직,이선화두속3소협,밀피유모,협표피세포수주벽천파상,본문지지타문위량개독립적속적분류처리.
Nineteen leaf epidermis samples representing six species in Apios and two in Cochlianthus were examined for the first time using both light microscopy and scanning electron microscopy.Leaf epidermal characters of these two closely related genera,such as shape of cells,pattern of anticlinal walls,cuticular membrane and wax ornamentation,are usually stable within species and thus of great significance in understanding the relationships between and within genera.The results indicate that(1)A.gracillima Dunn should be recognized as a distinct species,not a variety of Apios delavayi Franch.,(2)the division of Apios into two subgenera is unreasonable,and(3)Apios and Cochlianthus are retained as two distinct genera.