CAJ | 학술논문

采用半补齐方法建立棉铃虫多核衣壳型多角体病毒基因组文库,通过对插入片段进行克隆鉴定和序列分析,获得了38k基因.该基因上游具有晚期调控保守序列TTAAG,是一个晚期表达基因,基因阅读框为903 bp,共编码300个氨基酸,氨基酸序列同源性分析结果表明其与α类杆状病毒的同源性较高,有较近的亲缘关系.氨基酸高级结构的分析表明其与与磷酸酶结构相似性达到95%,与病毒核衣壳的组装有关.
채용반보제방법건립면령충다핵의각형다각체병독기인조문고,통과대삽입편단진행극륭감정화서렬분석,획득료38k기인.해기인상유구유만기조공보수서렬TTAAG,시일개만기표체기인,기인열독광위903 bp,공편마300개안기산,안기산서렬동원성분석결과표명기여α류간상병독적동원성교고,유교근적친연관계.안기산고급결구적분석표명기여여린산매결구상사성체도95%,여병독핵의각적조장유관.
A genomic library of Helicoverpa armigera multiple nucleocapsid nucleopolyhedrovirus(HearMNPV)was constructed by"partial filling-in" method,the inserted sequence showed high identity to that the 38k gene was identified and acquired,its open reading frame(ORF)has 903 base pairs which encoded 300 amino acids.It has a late gene motif TTAAG and behaves similarly to all baculovirus late gene.The amino acid sequence analysis showed that the HearMNPV 38K protein has higher identities with Alphabaculovirus and has more closer relationship with Alphabaculovirus.The prediction of 38K Protein tertiary structure showed its structure has 95% identity with that of phosphatase,and it was related with nucleocapsid assembling.