食品科学
食品科學
식품과학
FOOD SCIENCE
2010年
19期
329-334
,共6页
枸杞多糖%人宫颈癌 Hela 细胞%凋亡%形态学
枸杞多糖%人宮頸癌 Hela 細胞%凋亡%形態學
구기다당%인궁경암 Hela 세포%조망%형태학
Lycium barbarum polysaccharides%human cervical carcinoma Hela cells%apoptosis%morphology
采用热水法提取药材枸杞子中的枸杞多糖.本研究通过MTT实验发现3.125～200mg/L质量浓度范围内的枸杞多糖能显著抑制宫颈癌Hela细胞的增殖;采用荧光显微镜、透射电镜和激光共聚焦扣描显微镜观察发现经枸杞多糖处理过的Hela细胞呈现出典型的凋亡特征;原位末端脱氧核苷酸转移酶标记法进一步证实经枸杞多糖处理过的Hela细胞呈现凋亡特征.结果提示枸杞多糖是一种潜在的抗癌复合物,其关键作用机制是诱导癌细胞凋亡.
採用熱水法提取藥材枸杞子中的枸杞多糖.本研究通過MTT實驗髮現3.125～200mg/L質量濃度範圍內的枸杞多糖能顯著抑製宮頸癌Hela細胞的增殖;採用熒光顯微鏡、透射電鏡和激光共聚焦釦描顯微鏡觀察髮現經枸杞多糖處理過的Hela細胞呈現齣典型的凋亡特徵;原位末耑脫氧覈苷痠轉移酶標記法進一步證實經枸杞多糖處理過的Hela細胞呈現凋亡特徵.結果提示枸杞多糖是一種潛在的抗癌複閤物,其關鍵作用機製是誘導癌細胞凋亡.
채용열수법제취약재구기자중적구기다당.본연구통과MTT실험발현3.125～200mg/L질량농도범위내적구기다당능현저억제궁경암Hela세포적증식;채용형광현미경、투사전경화격광공취초구묘현미경관찰발현경구기다당처리과적Hela세포정현출전형적조망특정;원위말단탈양핵감산전이매표기법진일보증실경구기다당처리과적Hela세포정현조망특정.결과제시구기다당시일충잠재적항암복합물,기관건작용궤제시유도암세포조망.
Lycium barbarum polysaccharides (LBP), isolated with boiling water from Lycium barbarum fruits, a famous Chinese medicinal herb, is one of the most important functional constituents in Lycium barbarum. In this study, the effect of LBP at the range of 3.125 to 200 mg/L on the proliferation of Hela cells was measured. After Hela cells were treated with LBP, typical apoptotic morphological changes were observed by fluorescence microscope, transmission electron microscope (TEM) and laser scanning confocal microscope (LSCM). Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay also confirmed that LBP-treated Hela cells showed apoptotic features. The results suggest that LBP is a potential antiumor compound.
