CAJ | 학술논문

目的在碳化二亚胺交联的去细胞牛心包膜上采用3种方式进行肝素改性,即共价键合肝素、离子结合肝素以及共价-离子联合负载肝素,比较这3种肝素化方式对牛心包膜抗凝血性能的影响,并筛选出最佳肝素化方式.方法通过溶血试验、血小板黏附实验、体外凝血测试以及复钙时间测定观察3种方式肝素化的基质抗凝血性能和血栓形成情况,以评价其血液相容性.结果综合上述4项检测的结果,共价-离子联合负载肝素改性的抗凝血性能优于共价键合肝素、离子结合肝素这2种单独负载肝素方式,溶血率<5%,电镜照片无血小板黏附,体外生理盐水浸泡15d后,仍保持良好的抗凝血活性.结论共价-离子联合负载肝素,将离子结合肝素活性好、共价键合肝素稳定性强的优点结合起来,取长补短,使这种方式肝素改性的牛心包具有良好的血液相容性.
목적 재탄화이아알교련적거세포우심포막상채용3충방식진행간소개성,즉공개건합간소、리자결합간소이급공개-리자연합부재간소,비교저3충간소화방식대우심포막항응혈성능적영향,병사선출최가간소화방식.방법 통과용혈시험、혈소판점부실험、체외응혈측시이급복개시간측정관찰3충방식간소화적기질항응혈성능화혈전형성정황,이평개기혈액상용성.결과 종합상술4항검측적결과,공개-리자연합부재간소개성적항응혈성능우우공개건합간소、리자결합간소저2충단독부재간소방식,용혈솔<5%,전경조편무혈소판점부,체외생리염수침포15d후,잉보지량호적항응혈활성.결론 공개-리자연합부재간소,장리자결합간소활성호、공개건합간소은정성강적우점결합기래,취장보단,사저충방식간소개성적우심포구유량호적혈액상용성.
Objective To investigate the anticoagulant properties of heparinized bovine pericardiums and make the best choice among three ways of heparinization: Heparinized EDC treated bovine pericardiums; Heparinized PEI treated bovine pericardiums; Heparinized EDC-PEI treated bovine pericardiums. Methods The blood compatibility of heparinized bovine pericardiums was assessed in vitro by hemolysis test, platelet attachment test, PT/APTT and recalcification time. Results Heparinized EDC-PEI treated bovine pericardiums were the best in terms of the anticoagulant properties, which had low hemolysis ratio(<5%) and no platelet attachment, even that PT/APTT and recalcification time were prolonged obviously for 15 days. Conclusion Heparinized EDC-PEI treated bovine pericardiums have stable and promising blood compatibility.