CAJ | 학술논문

目的探讨西洋参茎叶皂苷对大鼠局灶性脑缺血损伤的保护作用及其作用机制.方法采用大鼠大脑中动脉内栓线阻断法制备局灶性脑缺血损伤模型,通过化学比色法、硫代巴比妥酸法检测西洋参茎叶皂苷对脑缺血损伤大鼠脑组织乳酸(LA)、乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)四个生化指标的影响.结果西洋参茎叶皂苷(100、50 mg/kg)可明显降低LA、MDA含量及提高LDH、SOD活性.结论西洋参茎叶皂苷对大鼠局灶性脑缺血损伤具有保护作用,其作用机制可能与抑制酸中毒、对抗自由基和拮抗过氧化损伤有关.
목적 탐토서양삼경협조감대대서국조성뇌결혈손상적보호작용급기작용궤제.방법 채용대서대뇌중동맥내전선조단법제비국조성뇌결혈손상모형,통과화학비색법、류대파비타산법검측서양삼경협조감대뇌결혈손상대서뇌조직유산(LA)、유산탈경매(LDH)、병이철(MDA)、초양화물기화매(SOD)사개생화지표적영향.결과 서양삼경협조감(100、50 mg/kg)가명현강저LA、MDA함량급제고LDH、SOD활성.결론 서양삼경협조감대대서국조성뇌결혈손상구유보호작용,기작용궤제가능여억제산중독、대항자유기화길항과양화손상유관.
Objective To investigate the protective effct of Panax quinquefolium saponins from steams and leaves(PQS)on focal cerebral ischemia injury in rats and its mechanisms. Methods Wistar rats were randomly divided into sham operation group,model control group,nimodipine group and two PQS groups,in which PQS of 100 and 50 mg/kg was intragastrically administered. Focal cerebral ischemia model was established by middle cerebral artery occlusion (MCAO)in rats, via string ligation of artetia carotis interna. The content of malondicldehy de(MDA) was determined by thibabituric acid ( TBA ) test, the activity of lactate dehydrogenase ( LDH ), superoxide dismutase (SOD)and the content of lactic acid(LA) were detected by chemical colorimetry test in cerebral tissues. Results PQS( 100,50mg/kg)could significantly decrease the content of LA、MDA and increase the activity of LDH、SOD. Conclusion The protective mechanism of PQS on focal cerebral ischemia injury may be related to reduce acidosis, anti-free radical and resist oxidative damage.