国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
2期
107-110
,共4页
刘远新%刘婷%杨晓燕%李卉%张云桥%李惠武%刘佳%孟新玲%姜涛%刘丰%王小鲁
劉遠新%劉婷%楊曉燕%李卉%張雲橋%李惠武%劉佳%孟新玲%薑濤%劉豐%王小魯
류원신%류정%양효연%리훼%장운교%리혜무%류가%맹신령%강도%류봉%왕소로
安宫牛黄丸%脑出血%eNOSmRNA
安宮牛黃汍%腦齣血%eNOSmRNA
안궁우황환%뇌출혈%eNOSmRNA
Angong-niuhuang Pills%Intracerebral hemorrhage%eNOSmRNA
目的 观察安宫牛黄丸对自发性高血压大鼠脑出血后内皮型一氧化氮合酶信使核糖核酸(eNOSm RNA)表达的影响,探讨其保护作用机制.方法 采用自体血制作大鼠脑出血模型,将120只自发性高血压大鼠按随机数字表法分为模型组、西药治疗组、安宫牛黄丸组、中西药结合组.应用RT-PCR法测定用药后eNOSmRNA表达的变化.结果 中西结合组eNOSmRNA表达明显增高(P＜0.01),安宫牛黄组、西药治疗组eNOSmRNA表达明显高于模型组(P＜0.01),安宫牛黄丸组与两药治疗组间相比差异无统计学意义(P＞0.05).结论 安宫牛黄丸对自发性高血压大鼠脑出血后的治疗作用可能与eNOS的表达有关.
目的 觀察安宮牛黃汍對自髮性高血壓大鼠腦齣血後內皮型一氧化氮閤酶信使覈糖覈痠(eNOSm RNA)錶達的影響,探討其保護作用機製.方法 採用自體血製作大鼠腦齣血模型,將120隻自髮性高血壓大鼠按隨機數字錶法分為模型組、西藥治療組、安宮牛黃汍組、中西藥結閤組.應用RT-PCR法測定用藥後eNOSmRNA錶達的變化.結果 中西結閤組eNOSmRNA錶達明顯增高(P＜0.01),安宮牛黃組、西藥治療組eNOSmRNA錶達明顯高于模型組(P＜0.01),安宮牛黃汍組與兩藥治療組間相比差異無統計學意義(P＞0.05).結論 安宮牛黃汍對自髮性高血壓大鼠腦齣血後的治療作用可能與eNOS的錶達有關.
목적 관찰안궁우황환대자발성고혈압대서뇌출혈후내피형일양화담합매신사핵당핵산(eNOSm RNA)표체적영향,탐토기보호작용궤제.방법 채용자체혈제작대서뇌출혈모형,장120지자발성고혈압대서안수궤수자표법분위모형조、서약치료조、안궁우황환조、중서약결합조.응용RT-PCR법측정용약후eNOSmRNA표체적변화.결과 중서결합조eNOSmRNA표체명현증고(P＜0.01),안궁우황조、서약치료조eNOSmRNA표체명현고우모형조(P＜0.01),안궁우황환조여량약치료조간상비차이무통계학의의(P＞0.05).결론 안궁우황환대자발성고혈압대서뇌출혈후적치료작용가능여eNOS적표체유관.
Objective To study the effect of Angong-niuhuang pill on expression of eNOSmRNA in spontaneously hypertensive rats after intracerebral hemorrhage and to explore it's protective mechanism.Methods 120 spontaneously hypertensive rats were randomly divided into four groups: model group, western medicine treated group. Angong-niuhuang pill treated group, combining traditional Chinese medicine western medicine group, expression of eNOSmRNA in rat brain tissue was detected by RT-PCR. Results Expression of BCL-2mRNA in combining traditional Chinese medicine western medicine group was significantly higer than other goups(P＜0.01), Compared with model group, the expression of BCL-2mRNA in western medicine treated group and Angong-niuhuang pill treated group was markedly increased (P＜0.01), the differences between Angong-niuhuang pill treated group and Western medicine treated group were not obvious (P＞0.05).Conclusion Protective effect of Angong-niuhuang pill on spontaneously hypertensive rats after intracerebral hemorrhage is related to the the inducement of eNOS expression.
