CAJ | 학술논문

背景骨桥蛋白(OPN)在多种转移性肿瘤组织中表达增高,但在葡萄膜黑色素瘤(UM)中的表达与临床病理特征及侵袭转移是否相关尚不清楚. 目的研究UM组织及不同转移潜能人UM细胞系MUM-2B、C918和OCM-1A中OPN的表达情况,分析OPN的组织及细胞系表达水平与UM患者临床病理特征与转移预后之间的关系.方法收集2004年1月至2007年12月在北京同仁医院行眼球摘除手术并已经病理证实为脉络膜黑色素瘤组织的标本共50例,应用免疫组织化学法检测石蜡标本中OPN的表达,分析其与临床资料的相关性.应用定量聚合酶链反应(Q-PCR)技术检测不同侵袭性转移潜能的人UM细胞系中OPN mRNA的表达情况.结果 50例UM组织中发生肝转移者13例,其中10例OPN表达阳性,未发生肝转移的37例中14例OPN表达阳性；上皮型与非上皮型脉络膜黑色素瘤OPN表达阳性者分别为11/15和13/35；肿瘤累及睫状体和未累及者OPN表达阳性者分别为20/30和4/20；上述指标的比较差异均有统计学意义(X2=5.888、5.510、10.470,P＜0.05).患者不同性别、年龄、眼别、肿瘤最大基底径以及是否侵犯巩膜导管间OPN表达阳性例数的比较差异均无统计学意义(P=0.536、0.256、0.802、0.848、0.555).转移潜能细胞系由高到低依次为MUM-2B、C918、OCM-1A,其OPN mRNA相对表达量分别为1.00±0.04、0.91±0.03、0.08±0.01,差异有统计学意义(F=33.135,P＜0.05),MUM-2B、C918中OPN mRNA的表达水平较OCM-1A中明显增高,差异均有统计学意义(P=0.00),而MUM-2B、C918中OPN mRNA的表达水平差异无统计学意义(P=0.804).结论 OPN与UM侵袭能力及转移潜能密切相关,发生转移的UM组织及高侵袭转移性细胞系中OPN表达水平明显升高,提示OPN可能作为预测UM侵袭能力、转移潜能以及患者预后的指标.
배경 골교단백(OPN)재다충전이성종류조직중표체증고,단재포도막흑색소류(UM)중적표체여림상병리특정급침습전이시부상관상불청초. 목적 연구UM조직급불동전이잠능인UM세포계MUM-2B、C918화OCM-1A중OPN적표체정황,분석OPN적조직급세포계표체수평여UM환자림상병리특정여전이예후지간적관계.방법 수집2004년1월지2007년12월재북경동인의원행안구적제수술병이경병리증실위맥락막흑색소류조직적표본공50례,응용면역조직화학법검측석사표본중OPN적표체,분석기여림상자료적상관성.응용정량취합매련반응(Q-PCR)기술검측불동침습성전이잠능적인UM세포계중OPN mRNA적표체정황.결과 50례UM조직중발생간전이자13례,기중10례OPN표체양성,미발생간전이적37례중14례OPN표체양성；상피형여비상피형맥락막흑색소류OPN표체양성자분별위11/15화13/35；종류루급첩상체화미루급자OPN표체양성자분별위20/30화4/20；상술지표적비교차이균유통계학의의(X2=5.888、5.510、10.470,P＜0.05).환자불동성별、년령、안별、종류최대기저경이급시부침범공막도관간OPN표체양성례수적비교차이균무통계학의의(P=0.536、0.256、0.802、0.848、0.555).전이잠능세포계유고도저의차위MUM-2B、C918、OCM-1A,기OPN mRNA상대표체량분별위1.00±0.04、0.91±0.03、0.08±0.01,차이유통계학의의(F=33.135,P＜0.05),MUM-2B、C918중OPN mRNA적표체수평교OCM-1A중명현증고,차이균유통계학의의(P=0.00),이MUM-2B、C918중OPN mRNA적표체수평차이무통계학의의(P=0.804).결론 OPN여UM침습능력급전이잠능밀절상관,발생전이적UM조직급고침습전이성세포계중OPN표체수평명현승고,제시OPN가능작위예측UM침습능력、전이잠능이급환자예후적지표.
Background Osteopontin is highly expressed in many metastatic tumors,but its expression in uveal melanoma and the relationship of osteopontin with metastasis is still under investigation. Objective This study was to investigate the relationship between osteopontin expression and uveal melanoma metastatic potential.Methods Fifty samples of uveal melanoma were collected in Beijing Tongren Hospital.The expressions of osteopontin in the samples were detected using immunohistochemistry,and expressions of osteopontin mRNA in different metastatic potential uveal melanoma cell lines (MU M-2B,C918,OCM-1A) were analyzed using quantitative polymerase chain reaction (Q-PCR). Results Osteopontin was positively expressed in 10 samples of 13 patients with metastasis and 14 samples of 37 patients without metastasis,showing a significant difference between them (x2=5.888,P=0.000).There were 11 samples and 13 samples exhibiting the positive expression of osteopontin in 15 epithelial type and 35 non-epithelial type of uveal melanoma respectively with the significant difference (x2=5.510,P =0.000).The positive expressing numbers of osteopontin were 20 tumors of 30 affected tumors and 4 tumors of 20unaffected ciliary tumors with the significant difference (x2 =10.470,P =0.000 ).No significant differences in the expression of osteopontige were found between different gender,age,eyes,largest basal diameters and invasion of scleral canal( P =0.536,0.256,0.802,0.848,0.555 ).The relative expression value of osteopontin mRNA was in turn 1.00±0.04,0.91 ±0.03,0.08±0.01 in MUM-2B,C918,OCM-1A with the significant difference among three cell lines( F=33.135,P＜0.05).The osteopontige mRNA expression was significant enhanced in high metastatic potentialcell lines( MUM-2B,C918 ) compared with low metastatic potential cell lines( OCM-1 A) (P＜0.05 ),but no significant difference was seen between two high metastatic potential cell lines(MUM-2B,C918) (P=0.804). Conclusions Osteopontige expression is associated with the uveal melanoma cell line invasion and metastasis potential.These results imply that osteopontige expression maybe an indicator of uveal melanoma invasion and metastasis ability.